Over-expression of recombinant human interferon-gamma in high cell density fermentation of Escherichia coli

被引：48

作者：

Khalilzadeh, R ^{[1
]}

Shojaosadati, SA ^{[1
]}

Bahrami, A ^{[1
]}

Maghsoudi, N ^{[1
]}

机构：

[1] Tarbiat Modarres Univ, Fac Engn, Dept Chem Engn, Biotechnol Grp, Tehran, Iran

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 23期

关键词：

Escherichia coli; fed-batch fermentation; plasmid stability; recombinant human interferon-gamma;

D O I：

10.1023/B:BILE.0000004390.98648.25

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human interferon-gamma (hIFN-gamma) was expressed in Escherichia coli BL21(DE3) under the control of the T7 promoter. Glucose was used as the sole source of carbon and energy with simple exponential feeding rate in fed-batch process. Cell density of recombinant E. coli was reached to 100 g dry wt l(-1) under both constant (0.12 h(-1)) and variable (0.12 - 0.52 h(-1)) specific growth rates. In the variable specific growth rate fed-batch process, plasmid stability and specific yield of rhIFN-gamma were greater than constant specific growth rate fed-batch process. The final specific yield and overall productivity of rhIFN-gamma were 0.35 +/- 0.02 g rhIFN-gamma g(-1) dry cell wt and 0.9 +/- 0.05 g rhIFN-gamma l(-1) h(-1) in the variable specific growth rate fed-batch process, respectively.

引用

页码：1989 / 1992

页数：4