Autographic Assay for the Rapid Detection of Antioxidant Capacity of Liquid and Semi-solid Pharmaceutical Formulations Using ABTS•+ Immobilized by Gel Entrapment

被引:32
|
作者
Zampini, Iris Catiana
Ordonez, Roxana Mabel
Isla, Maria Ines [1 ]
机构
[1] Univ Nacl Tucuman, Fac Ciencias Nat, CONICET, INQUINOA,Fac Bioquim Quim & Farm, RA-4000 San Miguel De Tucuman, Argentina
来源
AAPS PHARMSCITECH | 2010年 / 11卷 / 03期
关键词
ABTS(center dot+); antioxidant activity; pharmaceutical formulations; plant extract; TLC-autographic method; RADICAL-SCAVENGING ACTIVITY; ANTIBACTERIAL ACTIVITY;
D O I
10.1208/s12249-010-9484-y
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
An autographic assay suitable for the detection of antioxidant compounds in a complex matrix (liquid and semi-solid pharmaceutical formulations) or in isolated compounds was described. The preformed radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)) was generated by oxidation of ABTS with potassium persulfate and reduced in the presence of hydrogen-donating antioxidants. For a further comparative estimation of its applicability and sensitivity, different medicinal plant extracts, hydrogels and antioxidant compounds were dot seeded or chromatographed on silica gel (TLC) and revealed with ABTS(center dot+) solution (System I) or ABTS(center dot+) immobilized by gel entrapment (System II). Both systems were effective and able to detect antioxidant activity in a micromolar range in seconds. System II was more sensitive and reproducible than System I. This micromethod is quick, inexpensive, and particularly helpful whether it works with numerous samples or on a small scale.
引用
收藏
页码:1159 / 1163
页数:5
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