Autographic Assay for the Rapid Detection of Antioxidant Capacity of Liquid and Semi-solid Pharmaceutical Formulations Using ABTS•+ Immobilized by Gel Entrapment
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Zampini, Iris Catiana
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机构:Univ Nacl Tucuman, Fac Ciencias Nat, CONICET, INQUINOA,Fac Bioquim Quim & Farm, RA-4000 San Miguel De Tucuman, Argentina
Zampini, Iris Catiana
Ordonez, Roxana Mabel
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机构:Univ Nacl Tucuman, Fac Ciencias Nat, CONICET, INQUINOA,Fac Bioquim Quim & Farm, RA-4000 San Miguel De Tucuman, Argentina
Ordonez, Roxana Mabel
Isla, Maria Ines
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Univ Nacl Tucuman, Fac Ciencias Nat, CONICET, INQUINOA,Fac Bioquim Quim & Farm, RA-4000 San Miguel De Tucuman, ArgentinaUniv Nacl Tucuman, Fac Ciencias Nat, CONICET, INQUINOA,Fac Bioquim Quim & Farm, RA-4000 San Miguel De Tucuman, Argentina
Isla, Maria Ines
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[1] Univ Nacl Tucuman, Fac Ciencias Nat, CONICET, INQUINOA,Fac Bioquim Quim & Farm, RA-4000 San Miguel De Tucuman, Argentina
An autographic assay suitable for the detection of antioxidant compounds in a complex matrix (liquid and semi-solid pharmaceutical formulations) or in isolated compounds was described. The preformed radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)) was generated by oxidation of ABTS with potassium persulfate and reduced in the presence of hydrogen-donating antioxidants. For a further comparative estimation of its applicability and sensitivity, different medicinal plant extracts, hydrogels and antioxidant compounds were dot seeded or chromatographed on silica gel (TLC) and revealed with ABTS(center dot+) solution (System I) or ABTS(center dot+) immobilized by gel entrapment (System II). Both systems were effective and able to detect antioxidant activity in a micromolar range in seconds. System II was more sensitive and reproducible than System I. This micromethod is quick, inexpensive, and particularly helpful whether it works with numerous samples or on a small scale.
机构:Universidad Nacional de Tucumán,INQUINOA. CONICET. Facultad de Bioquímica, Química y Farmacia. Facultad de Ciencias Naturales e Instituto Miguel Lillo
Iris Catiana Zampini
Roxana Mabel Ordoñez
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机构:Universidad Nacional de Tucumán,INQUINOA. CONICET. Facultad de Bioquímica, Química y Farmacia. Facultad de Ciencias Naturales e Instituto Miguel Lillo
Roxana Mabel Ordoñez
María Inés Isla
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机构:Universidad Nacional de Tucumán,INQUINOA. CONICET. Facultad de Bioquímica, Química y Farmacia. Facultad de Ciencias Naturales e Instituto Miguel Lillo