Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae)

被引：70

作者：

Torres-Rego, Manoela ^{[1
]}

Furtado, Allanny Alves ^{[1
]}

Oliveira Bitencourt, Mariana Angelica ^{[1
]}

Jeronimo de Souza Lima, Maira Conceicao ^{[1
]}

Lisboa Castro de Andrade, Rafael Caetano ^{[1
]}

de Azevedo, Eduardo Pereira ^{[2
]}

Soares, Thaciane da Cunha ^{[3
]}

Tomaz, Jose Carlos ^{[4
]}

Lopes, Norberto Peporine ^{[4
]}

da Silva-Junior, Arnobio Antonio ^{[1
]}

Zucolotto, Silvana Maria ^{[3
]}

Fernandes-Pedrosa, Matheus de Freitas ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Norte, Ctr Ciencias Saude, Fac Farm, Dept Farm,Lab Tecnol & Biotecnol Farmaceut TecBio, Rua Gal Gustavo Cordeiro de Farias S-N, BR-59012570 Natal, RN, Brazil

[2] Univ Potiguar, Dept Posgrad Biotecnol, Ave Senador Salgado Filho 1610, BR-59056000 Natal, RN, Brazil

[3] Univ Fed Rio Grande do Norte, Ctr Ciencias Saude, Fac Farm, Dept Farm,Lab Farmacognosia, Rua Gal Gustavo Cordeiro de Farias S-N, BR-59012570 Natal, RN, Brazil

[4] Univ Sao Paulo, NPPNS, Dept Quim & Fis, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP, Brazil

来源：

BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2016年 / 16卷

关键词：

Anti-inflammatory; Apocynaceae; Chlorogenic acid; Hancornia speciosa; Mangabeira; Rutin; INDUCED INFLAMMATORY RESPONSE; MEDICINAL-PLANTS; NEUTROPHIL MIGRATION; CARRAGEENAN; VASODILATION; INVOLVEMENT; INHIBITION; ACTIVATION; RECEPTOR; EDEMA;

D O I：

10.1186/s12906-016-1259-x

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Background: Hancornia speciosa Gomes (Apocynaceae), popularly known as "mangabeira," has been used in folk medicine to treat inflammatory disorders, hypertension, dermatitis, diabetes, liver diseases and gastric disorders. Although the ethnobotany indicates that its fruits can be used for the treatment of ulcers and inflammatory disorders, only few studies have been conducted to prove such biological activities. This study investigated the anti-inflammatory properties of the aqueous extract of the fruits of H. speciosa Gomes as well as its bioactive compounds using in vivo experimental models. Methods: The bioactive compounds were identified by High Performance Liquid Chromatography coupled with diode array detector (HPLC-DAD) and Liquid Chromatography coupled with Mass Spectrometry (LC-MS). The anti-inflammatory properties were investigated through in vivo tests, which comprised xylene-induced ear edema, carrageenan-induced peritonitis and zymosan-induced air pouch. The levels of IL-1 beta, IL-6, IL-12 and TNF-alpha were determined using ELISA. Results: Rutin and chlorogenic acid were identified in the extract as the main secondary metabolites. In addition, the extract as well as rutin and chlorogenic acid significantly inhibited the xilol-induced ear edema and also reduced the cell migration in both carrageenan-induced peritonitis and zymosan-induced air pouch models. Reduced levels of cytokines were also observed. Conclusion: This is the first study that demonstrated the anti-inflammatory activity of the extract of H. speciosa fruits against different inflammatory agents in animal models, suggesting that its bioactive molecules, especially rutin and chlorogenic acid are, at least in part, responsible for such activity. These findings support the widespread use of Hancornia speciosa in popular medicine and demonstrate that its aqueous extract has therapeutical potential for the development of herbal drugs with anti-inflammatory properties.

引用

页数：10

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