In vitro properties of small ruminant lentivirus genotype E

被引:16
作者
Juganaru, M. [1 ]
Reina, R. [2 ]
Bertolotti, L. [1 ]
Stella, M. C. [1 ]
Profiti, M. [1 ]
Armentano, M. [3 ]
Bollo, E. [4 ]
Amorena, B. [2 ]
Rosati, S. [1 ]
机构
[1] Univ Turin, Dipartimento Prod Anim Epidemiol & Ecol, I-10095 Grugliasco, TO, Italy
[2] Univ Publ Navarra, CSIC, Inst Agrobiotecnol, Navarra, Spain
[3] Univ Turin, Dipartimento Morfofisiol Vet, I-10095 Grugliasco, TO, Italy
[4] Univ Turin, Dipartimento Patol Anim, I-10095 Grugliasco, TO, Italy
关键词
Small ruminant lentivirus; Genotype E; dUTPase; vpr-like; Viral pseudotype; Entry assay; CAPRINE-ARTHRITIS-ENCEPHALITIS; MAEDI-VISNA-VIRUS; HUMAN-IMMUNODEFICIENCY-VIRUS; URACIL-DNA GLYCOSYLASE; PHYLOGENETIC ANALYSIS; NATURAL TRANSMISSION; OVINE LENTIVIRUSES; SHEEP RETROVIRUS; VIRAL PARTICLES; MIXED FLOCKS;
D O I
10.1016/j.virol.2010.10.031
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Small ruminant lentivirus genotype E lacks the dUTPase subunit and vpr-like gene. Two strains (Roccaverano and Seui) with identical genetic organization have been described, with the env HV1-HV2 domains being the most divergent. Although dUTPase and vpr-like deletions have been involved in the RT fidelity in non dividing cells, both strains were able to replicate efficiently in blood derived macrophages (BDM), while virus production of El subtype was reduced or abrogated in replicating fibroblastic-like cells. The transcriptional activity of genotype E was similar in these two cellular populations. When viral pseudotypes were generated with the env of both viruses, Roccaverano pseudotype displayed a paranuclear localization on BDM, suggesting a different mechanism of entry. Polymorphic GAS and TAS sites in the U3 region, further suggest that a population different from classically activated macrophages can be infected by these viruses, opening new insights into lentiviruses with low or null pathogenic potential. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:88 / 95
页数:8
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