Effects of Zinc Chelators on Aflatoxin Production in Aspergillus parasiticus

被引:9
作者
Wee, Josephine [1 ,3 ,4 ]
Day, Devin M. [1 ]
Linz, John E. [1 ,2 ,3 ]
机构
[1] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA
[2] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA
[3] Michigan State Univ, Ctr Integrat Toxicol, E Lansing, MI 48824 USA
[4] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA
来源
TOXINS | 2016年 / 8卷 / 06期
关键词
Aspergillus parasiticus; aflatoxin biosynthesis; zinc; zinc chelators; SECONDARY METABOLISM; OXIDATIVE STRESS; BIOSYNTHESIS; EXPRESSION; CONTAMINATION; PATHWAY; FLAVUS; GENES; RESISTANCE; DISTINCT;
D O I
10.3390/toxins8060171
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Zinc concentrations strongly influence aflatoxin accumulation in laboratory media and in food and feed crops. The presence of zinc stimulates aflatoxin production, and the absence of zinc impedes toxin production. Initial studies that suggested a link between zinc and aflatoxin biosynthesis were presented in the 1970s. In the present study, we utilized two zinc chelators, N,N,N',N'-tetrakis (2-pyridylmethyl) ethane-1,2-diamine (TPEN) and 2,3-dimercapto-1-propanesulfonic acid (DMPS) to explore the effect of zinc limitation on aflatoxin synthesis in Aspergillus parasiticus. TPEN but not DMPS decreased aflatoxin biosynthesis up to six-fold depending on whether A. parasiticus was grown on rich or minimal medium. Although we observed significant inhibition of aflatoxin production by TPEN, no detectable changes were observed in expression levels of the aflatoxin pathway gene ver-1 and the zinc binuclear cluster transcription factor, AflR. Treatment of growing A. parasiticus solid culture with a fluorescent zinc probe demonstrated an increase in intracellular zinc levels assessed by increases in fluorescent intensity of cultures treated with TPEN compared to controls. These data suggest that TPEN binds to cytoplasmic zinc therefore limiting fungal access to zinc. To investigate the efficacy of TPEN on food and feed crops, we found that TPEN effectively decreases aflatoxin accumulation on peanut medium but not in a sunflower seeds-derived medium. From an application perspective, these data provide the basis for biological differences that exist in the efficacy of different zinc chelators in various food and feed crops frequently contaminated by aflatoxin.
引用
收藏
页数:14
相关论文
共 45 条
[1]   REGULATION OF AFLATOXIN BIOSYNTHESIS - INDUCTION OF AFLATOXIN PRODUCTION BY VARIOUS CARBOHYDRATES [J].
ABDOLLAHI, A ;
BUCHANAN, RL .
JOURNAL OF FOOD SCIENCE, 1981, 46 (02) :633-635
[2]  
Aziz NH, 1997, NAHRUNG, V41, P150, DOI 10.1002/food.19970410307
[3]   VeA Is Associated with the Response to Oxidative Stress in the Aflatoxin Producer Aspergillus flavus [J].
Baidya, Sachin ;
Duran, Rocio M. ;
Lohmar, Jessica M. ;
Harris-Coward, Pamela Y. ;
Cary, Jeffrey W. ;
Hong, Sung-Yong ;
Roze, Ludmila V. ;
Linz, John E. ;
Calvo, Ana M. .
EUKARYOTIC CELL, 2014, 13 (08) :1095-1103
[4]   The Biofilm-Specific Antibiotic Resistance Gene ndvB Is Important for Expression of Ethanol Oxidation Genes in Pseudomonas aeruginosa Biofilms [J].
Beaudoin, Trevor ;
Zhang, Li ;
Hinz, Aaron J. ;
Parr, Christopher J. ;
Thien-Fah Mah .
JOURNAL OF BACTERIOLOGY, 2012, 194 (12) :3128-3136
[5]  
Blaurock-Busch E., 2014, BR J MED MED RES, V4, P1821
[6]   REGULATION OF AFLATOXIN BIOSYNTHESIS - EFFECT OF GLUCOSE ON ACTIVITIES OF VARIOUS GLYCOLYTIC-ENZYMES [J].
BUCHANAN, RL ;
LEWIS, DF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 48 (02) :306-310
[7]   Loss of human Greatwall results in G2 arrest and multiple mitotic defects due to deregulation of the cyclin B-Cdc2/PP2A balance [J].
Burgess, Andrew ;
Vigneron, Suzanne ;
Brioudes, Estelle ;
Labbe, Jean-Claude ;
Lorca, Thierry ;
Castro, Anna .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (28) :12564-12569
[8]   Zinc pyrithione induces ERK- and PKC-dependent necrosis distinct from TPEN-induced apoptosis in prostate cancer cells [J].
Carraway, Robert E. ;
Dobner, Paul R. .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2012, 1823 (02) :544-557
[9]   Regulatory elements in aflatoxin biosynthesis [J].
J. W. Cary ;
K. C. Ehrlich ;
S. P. Kale ;
A. M. Calvo ;
D. Bhatnagar ;
T. E. Cleveland .
Mycotoxin Research, 2006, 22 (2)
[10]   AFLATOXINS IN SUNFLOWER SEEDS - EFFECT OF ZINC IN AFLATOXIN PRODUCTION BY 2 STRAINS OF ASPERGILLUS-PARASITICUS [J].
CHULZE, S ;
FUSERO, S ;
DALCERO, A ;
ETCHEVERRY, M ;
VARSAVSKY, E .
MYCOPATHOLOGIA, 1987, 99 (02) :91-94