1H, 13C, and 15N resonance assignments of reduced GrxS14 from Populus tremula x tremuloides

被引:10
作者
Wang, Lei [2 ,3 ]
Ren, Xiaobai [2 ,3 ]
Li, Yifei [1 ,2 ]
Rouhier, Nicolas [4 ]
Jacquot, Jean-Pierre [4 ]
Jin, Changwen [1 ,2 ,3 ]
Xia, Bin [1 ,2 ,3 ]
机构
[1] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
[2] Peking Univ, Beijing Nucl Magnet Resonance Ctr, Beijing 100871, Peoples R China
[3] Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
[4] Univ Henri Poincare, IFR Genom Ecophysiol & Ecol Fonctionnelles 110, Unite Mixte Rech INRA UHP 1136, F-54506 Vandoeuvre Les Nancy, France
关键词
Glutaredoxin; NMR chemical shift assignments; Poplar; GLUTAREDOXINS;
D O I
10.1007/s12104-010-9282-1
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
GrxS14 is a monothiol Glutaredoxin (Grx) from Populus tremula x tremuloides, which has a CGFS active site. GrxS14 is located in the chloroplasts and has been found to occur ether as an apo form or as a holo form with a [2Fe-2S] cluster. The holo form contains two monomers of apo GrxS14 bridged by the iron sulphur center, in the presence of two external glutathione molecules (Bandyopadhyay et al. 2008). The NMR assignments of the GrxS14 are essential for its solution structure determination.
引用
收藏
页码:121 / 124
页数:4
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