Characterization and fine mapping of osh15(t), a novel dwarf mutant gene in rice (Oryza sativa L.)

被引:3
作者
Fan, Shuxiu [1 ,2 ]
Yao, Xiaoyun [1 ,2 ]
Liu, Jin [1 ,2 ]
Dong, Xiaoyan [1 ,2 ]
Mao, Ting [3 ]
Wang, Jiayu [1 ,2 ]
机构
[1] Shenyang Agr Univ, Key Lab Northeast Rice Biol & Breeding, Minist Agr, Shenyang 110866, Peoples R China
[2] Collaborat Innovat Ctr Genet Improvement & Qual &, Shenyang 110866, Peoples R China
[3] Liaoning Prov Saline & Alkaline Land Utilizat & R, Panjin 124010, Liaoning, Peoples R China
关键词
Rice; Dwarf mutant; Morphological characteristics; Giberellic acid; Fine mapping; HETEROTRIMERIC G-PROTEIN; SEMIDWARF GENE; ALPHA-SUBUNIT; PLANT HEIGHT; ELONGATION; BIOSYNTHESIS; PREVENTS; ENCODES; ENZYME; LOCUS;
D O I
10.1007/s13258-016-0430-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plant height is one of the most important agronomic traits of plant architecture, and also affects grain yield in rice. In this study, we obtained a novel dwarf rice mutant of japonica variety Shennong9816, designated Shennong9816d. Compared with wild-type, the Shennong9816d plant height was significantly reduced, and the tiller number significantly increased. Additionally, the mutant yield component, and the number of large and small vascular bundles were significantly decreased compared with wild-type. Genetic analysis indicated that the Shennong9816d dwarf phenotype was controlled by a recessive nuclear gene, while the plant was shown to be sensitive to gibberellic acid. Using a large F-2 population derived from a cross between Shennong9816d and the indica rice variety Habataki, the osh15(t) gene was fine mapped between RM20891 and RM20898, within a physical distance of 73.78 kb. Sequencing analysis showed that Shennong9816d carries a 1 bp mutation and a 30 bp insertion in the OSH15 region. These results suggest that osh15(t) is a novel allelic mutant originally derived from japonica variety Shennong9816, which may be useful for introducing the semi-dwarf phenotype to improve plant architecture in rice breeding practice.
引用
收藏
页码:849 / 856
页数:8
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