Agrobacterium-mediated transformation of cork oak (Quercus suber L.) somatic embryos

被引：9
|
作者：
Sánchez, N
Manzanera, JA
Pintos, B
Bueno, MA
机构：
[1] Inst Nacl Invest & Tecnol Agr & Alimentaria, Madrid 28040, Spain
[2] Univ Politecn Madrid, ETSI Montes, E-28040 Madrid, Spain
来源：
NEW FORESTS | 2005年 / 29卷 / 02期
关键词：
genetic transformation; hygromycin resistance; marker gene; reporter gene;
D O I：
10.1007/s11056-005-0208-1
中图分类号：
S7 [林业];
学科分类号：
0829 ; 0907 ;
摘要：
A simple method is presented for the unreported genetic transformation of cork oak (Quercus suber L.). Pro-embryo masses (PEMs) were induced on immature zygotic embryos applied to medium supplemented with 2.3 mu M 2,4-dichlorophenoxyacetic acid. The established PEMs were inoculated with Agrobacterium tumefaciens LBA4404/p35S GUS INT/pCAMBIA 1301 strain. Transformants were selected on hygromycin (IjYG) 94 mu M-supplemented medium. Viable embryos constituted 13% of those selected on HYG during 4 months. Expression of beta-glucuronidase at 4 months following co-cultivation confirmed transformation in 5.8% embryos selected on HYG. This method forms a basis for genetic transformation of cork oak somatic embryos.
引用
收藏
页码：169 / 176
页数：8
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