Isolation and Characterization of Shigella flexneri G3, Capable of Effective Cellulosic Saccharification under Mesophilic Conditions

被引：21

作者：

Wang, Aijie ^{[1
]}

Gao, Lingfang ^{[1
,3
]}

Ren, Nanqi ^{[1
]}

Xu, Jifei ^{[1
]}

Liu, Chong ^{[1
]}

Cao, Guangli ^{[1
]}

Yu, Hao ^{[1
,2
,3
]}

Liu, Wenzong ^{[1
,3
]}

Hemme, Christopher L. ^{[3
]}

He, Zhili ^{[3
]}

Zhou, Jizhong ^{[3
]}

机构：

[1] Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China

[2] Liaoning Tech Univ, Coll Resource & Environm Engn, Liaoning 123000, Peoples R China

[3] Univ Oklahoma, Dept Bot & Microbiol, Inst Environm Genom, Norman, OK 73019 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 02期

基金：

中国国家自然科学基金;

关键词：

CLOSTRIDIUM-CELLULOLYTICUM; HYDROGEN-PRODUCTION; MICROBIAL COMMUNITY; ACETIC-ACID; ETHANOL; DEGRADATION; FERMENTATION; HYDROLYSIS; BACTERIA; BIOMASS;

D O I：

10.1128/AEM.01230-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel Shigella strain (Shigella flexneri G3) showing high cellulolytic activity under mesophilic, anaerobic conditions was isolated and characterized. The bacterium is Gram negative, short rod shaped, and nonmotile and displays effective production of glucose, cellobiose, and other oligosaccharides from cellulose (Avicel PH-101) under optimal conditions (40 degrees C and pH 6.5). Approximately 75% of the cellulose was hydrolyzed in modified ATCC 1191 medium containing 0.3% cellulose, and the oligosaccharide production yield and specific production rate reached 375 mg g Avicel(-1) and 6.25 mg g Avicel(-1) h(-1), respectively, after a 60-hour incubation. To our knowledge, this represents the highest oligosaccharide yield and specific rate from cellulose for mesophilic bacterial monocultures reported so far. The results demonstrate that S. flexneri G3 is capable of rapid conversion of cellulose to oligosaccharides, with potential biofuel applications under mesophilic conditions.

引用

页码：517 / 523

页数：7

共 67 条

[1] Enhanced enzyme production from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger LMA grown as fed batch in a stirred tank bioreactor [J].

Ahamed, Aftab ;

Vermette, Patrick .

BIOCHEMICAL ENGINEERING JOURNAL, 2008, 42 (01) :41-46

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[3]

Baumann P., 1984, BERGEYS MANUAL SYSTE, P440

[4] Cellulose, cellulases and cellulosomes [J].

Bayer, EA ;

Chanzy, H ;

Lamed, R ;

Shoham, Y .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1998, 8 (05) :548-557

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6] Greenhouse gas production: A comparison between aerobic and anaerobic wastewater treatment technology [J].

Cakir, FY ;

Stenstrom, MK .

WATER RESEARCH, 2005, 39 (17) :4197-4203

[7] Acid hydrolysis of corn stover for biohydrogen production using Thermoanaerobacterium thermosaccharolyticum W16 [J].

Cao, Guangli ;

Ren, Nanqi ;

Wang, Aijie ;

Lee, Duu-Jong ;

Guo, Wanqian ;

Liu, Bingfeng ;

Feng, Yujie ;

Zhao, Qingliang .

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2009, 34 (17) :7182-7188

[8] CHARACTERIZATION OF CELLULOLYTIC ACTIVITIES IN COMMERCIAL TRICHODERMA-REESEI PREPARATIONS - AN APPROACH USING SMALL, CHROMOGENIC SUBSTRATES [J].

CLAEYSSENS, M ;

AERTS, G .

BIORESOURCE TECHNOLOGY, 1992, 39 (02) :143-146

[9] The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy [J].

Cole, JR ;

Chai, B ;

Marsh, TL ;

Farris, RJ ;

Wang, Q ;

Kulam, SA ;

Chandra, S ;

McGarrell, DM ;

Schmidt, TM ;

Garrity, GM ;

Tiedje, JM .

NUCLEIC ACIDS RESEARCH, 2003, 31 (01) :442-443

[10] Kinetics and metabolism of cellulose degradation at high substrate concentrations in steady-state continuous cultures of Clostridium cellulolyticum on a chemically defined medium [J].

Desvaux, M ;

Guedon, E ;

Petitdemange, H .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (09) :3837-3845

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