No Evidence of Cellular Alterations by MilliTesla-Level Static and 50 Hz Magnetic Fields on S-cerevisiae

被引:8
作者
Ruiz-Gomez, M. J. [1 ]
Ristori-Bogajo, E.
Prieto-Barcia, M. I. [2 ]
Martinez-Morillo, M.
机构
[1] Univ Malaga, Fac Med, Dept Radiol & Phys Med, Radiobiol Lab, E-29071 Malaga, Spain
[2] Univ Malaga, Dept Commun Engn, E-29071 Malaga, Spain
关键词
Magnetic field; Yeast; Survival; Cell cycle; Colony forming ability; Mutant; ELECTROMAGNETIC-FIELDS; ESCHERICHIA-COLI; GENE-EXPRESSION; EXPOSURE; CELLS; MUTATIONS; MT;
D O I
10.3109/07435800.2010.505158
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In an attempt to determine whether magnetic field (MF) exposures might induce cellular alterations, S. cerevisiae yeast cells were exposed to static or sinusoidal 50 Hz homogeneous MF (0.35 mT, 1.4 mT, and 2.45 mT) for 1 h and 72 h. Unsynchronized cells grown exponentially while exposed to MF, containing cells in all stages of the mitotic cell cycle. MF was generated by a pair of Helmholtz coils (40 cm in diameter, coaxial, separated by 20 cm). Survival, cell cycle distribution, colony forming ability, and mutation frequency were assayed. No differences in the above-mentioned parameters were observed in MF exposed samples in relation to unexposed controls, suggesting that homogeneous MF at these intensities do not produce appreciable cellular alterations in this organism under typical in vitro growth conditions.</.
引用
收藏
页码:154 / 164
页数:11
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