Comparative RNA-Seq Analysis of High- and Low-Oil Yellow Horn During Embryonic Development

被引:21
|
作者
Wang, Li [1 ]
Ruan, Chengjiang [1 ]
Liu, Lingyue [1 ]
Du, Wei [1 ]
Bao, Aomin [2 ]
机构
[1] Dalian Minzu Univ, Minist Educ, Inst Plant Resources, Key Lab Biotechnol & Bioresources Utilizat, Dalian 116600, Peoples R China
[2] Tongliao Acad Forestry Sci & Technol, Inst Econ Forest, Tongliao 028000, Peoples R China
基金
中国国家自然科学基金;
关键词
yellow horn; gene expression; RNA-seq; oil content; embryonic development; DIFFERENTIALLY EXPRESSED GENES; XANTHOCERAS-SORBIFOLIA BUNGE; ACETYL-COA CARBOXYLASE; SEED OIL; COMPARATIVE TRANSCRIPTOME; PROTEOMIC ANALYSIS; PALM FRUIT; IDENTIFICATION; ACCUMULATION; LINES;
D O I
10.3390/ijms19103071
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Yellow horn (Xanthoceras sorbifolium Bunge) is an endemic oil-rich shrub that has been widely cultivated in northern China for bioactive oil production. However, little is known regarding the molecular mechanisms that contribute to oil content in yellow horn. Herein, we measured the oil contents of high- and low-oil yellow horn embryo tissues at four developmental stages and investigated the global gene expression profiles through RNA-seq. The results found that at 40, 54, 68, and 81 days after anthesis, a total of 762, 664, 599, and 124 genes, respectively, were significantly differentially expressed between the high- and low-oil lines. Gene ontology (GO) enrichment analysis revealed some critical GO terms related to oil accumulation, including acyl-[acyl-carrier-protein] desaturase activity, pyruvate kinase activity, acetyl-CoA carboxylase activity, and seed oil body biogenesis. The identified differentially expressed genes also included several transcription factors, such as, AP2-EREBP family members, B3 domain proteins and C2C2-Dof proteins. Several genes involved in fatty acid (FA) biosynthesis, glycolysis/gluconeogenesis, and pyruvate metabolism were also up-regulated in the high-oil line at different developmental stages. Our findings indicate that the higher oil accumulation in high-oil yellow horn could be mostly driven by increased FA biosynthesis and carbon supply, i.e. a source effect.
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页数:18
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