Preferential expression and immunogenicity of HIV-1 Tat fusion protein expressed in tomato plant

被引：11

作者：

Cueno, Marni E. ^{[1
]}

Hibi, Yurina ^{[1
]}

Karamatsu, Katsuo ^{[2
]}

Yasutomi, Yasuhiro ^{[2
]}

Imai, Kenichi ^{[1
]}

Laurena, Antonio C. ^{[3
]}

Okamoto, Takashi ^{[1
]}

机构：

[1] Nagoya City Univ, Dept Mol & Cellular Biol, Mol & Cellular Biol Lab, Grad Sch Med Sci,Mizuho ku, Aichi 4678601, Japan

[2] Tsukuba Primate Res Ctr, Natl Inst Biomed Innovat, Lab Immunoregulat & Vaccine Res, Tsukuba, Ibaraki 3050843, Japan

[3] Univ Philippines Los Banos, Biochem Lab, Inst Plant Breeding, Laguna 4031, Philippines

来源：

TRANSGENIC RESEARCH | 2010年 / 19卷 / 05期

关键词：

AIDS; Antibody response; Cellular immune response; HIV-1; Tat; Transgenic tomato; T-CELL RESPONSES; AIDS VACCINE; INFECTION; GENES; PREVENTION; PARADIGM; BIOLOGY; HUMANS; SYSTEM; CIITA;

D O I：

10.1007/s11248-009-9358-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

HIV-1 Tat plays a major role in viral replication and is essential for AIDS development making it an ideal vaccine target providing that both humoral and cellular immune responses are induced. Plant-based antigen production, due to its cheaper cost, appears ideal for vaccine production. In this study, we created a plant-optimized tat and mutant (Cys30Ala/Lys41Ala) tat (mtat) gene and ligated each into a pBI121 expression vector with a stop codon and a gusA gene positioned immediately downstream. The vector construct was bombarded into tomato leaf calli and allowed to develop. We thus generated recombinant tomato plants preferentially expressing a Tat-GUS fusion protein over a Tat-only protein. In addition, plants bombarded with either tat or mtat genes showed no phenotypic difference and produced 2-4 mu g Tat-GUS fusion protein per milligram soluble plant protein. Furthermore, tomato extracts intradermally inoculated into mice were found to induce a humoral and, most importantly, cellular immunity.

引用

页码：889 / 895

页数：7

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