Biomarkers of pollution in soils irrigated with wastewater in Tunisia

Wastewater reuse for irrigation is expected in the Mediterranean basin as a sustainable alternative with socio-economic benefits However, wastewater carries considerable amounts of harmful chemicals and trace toxic metals that are able to accumulate in soil This pollution loading is threatening both the vegetation and the groundwater and, consequently, human health Therefore, in this study, in vitro bioassays were used to evaluate the adverse effects of wastewater and soil extract samples, on mammalian cells, with respect to heavy metal content (Ni, Cd, Pb, Fe and Al) The irrigation water, from a wastewater treatment plant, was sampled at Zaouit-Sousse perimeter in Tunisia A representative soil profile irrigated with wastewater (0-90 cm) and a non-irrigated profile (0-40 cm), serving as a control, were sampled in the olive-tree-occupied perimeter The estrogenic activity of the samples was investigated using the E-screen assay, while the heat shock protein (HSP) 47 assay aimed to determine the stress response of HSP 47-promoter-transfected cells following sample addition Furthermore, proteomics analyses were performed, aiming to establish specific biomarkers of environmental pollutants The irrigation water samples induced a high estrogenic activity when the MCF-7 cells were treated at 10% due to the presence of estrogenic compounds The E-screen assay revealed a cytotoxic effect of the surface soil samples (0-20 cm), even at low concentrations (0 001%) Furthermore, the proteomics experiments allowed the identification of specific biomarkers for estrogenicity and cytotoxicity on MCF-7 cells Moreover, significant stress response was observed following the incubations of the HSP 47-promoter-transfected cells with wastewater samples, even at 0 1%, and soil extracts (0-60 cm) at concentrations as low as 1% Interestingly, no stress response or cytotoxic effect was observed for the control soil or for deep layers below 60 cm These biological techniques could be extremely useful to evaluate the impact of wastewater reuse