Simultaneous quantitative determination of metronidazole and nalidixic acid in tablets by difference spectroscopy

被引:18
作者
Parimoo, P
Prasad, CVN
Vineeth, R
机构
[1] Department of Pharmacy, Birla Inst. of Technol. and Science, Pilani
关键词
metronidazole; nalidixic acid; difference spectroscopy; simultaneous quantitative determination;
D O I
10.1016/0731-7085(95)01659-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A difference spectrophotometric procedure has been developed for simultaneous determination of metronidazole (MDZ) and nalidixic acid (NA) in tablets. The method comprised the measurement of the absorbance of a solution of the tablet extract in 0.1 M NaOH relative to that of an equimolar solution in 0.1 M HCl at 292 nm for NA and 325 nm for MDZ. The presence of identical isosbestic points for pure drug solutions and tablet extracts indicated the non-interference of excipients in the absorption at these wavelengths. Compliance with Beer's law was observed in the concentration ranges 5-25 mu g ml(-1) for MDZ and 15-35 mu g ml(-1) for NA at these wavelengths.
引用
收藏
页码:389 / 393
页数:5
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