Immunostimulatory oligodeoxynucleotides induce dolphin neutrophil NADPH-oxidase activation in a CpG-independent but phosphorothioate backbone-dependent manner

被引：5

作者：

Itou, T

Endo, T

Sakai, T

Karlsson, A

Collins, LV

机构：

[1] Nihon Univ, Sch Vet Med, Dept Prevent Vet Med & Anim Hlth, Fujisawa, Kanagawa 2528510, Japan

[2] Shinagawa Aquarium, Shinagawa Ku, Tokyo 1400012, Japan

[3] Gothenburg Univ, Dept Rheumatol & Inflammat Res, S-41346 Gothenburg, Sweden

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2005年 / 29卷 / 07期

关键词：

dolphin; neutrophils; NADPH-oxidase; oligodeoxynucleotides; phosphorothioate backbone;

D O I：

10.1016/j.dci.2004.12.003

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Immunocytes. which include antigen-presenting cells, B cells, natural killer cells and neutrophils, can be stimulated directly or indirectly with bacterial DNA and synthetic oligodeoxynucleotides (ODNs) with different structures and sequences. In the present study. we investigated the effect of synthetic ODNs on the respiratory burst of dolphin neutrophils using a chemiluminescence assay. Phosphorothioate (PS)-ODNs dose-dependently induced the respiratory burst, while phosphodiester (PO)-ODNs did not, regardless of CpG-content. The PS-ODN-induced activity was completely abolished by the flavoprotein inhibitor diphenyleneiodonium, which indicates that the NADPH-oxidase is activated by PS-ODNs. These results reveal that PS-ODNs induce dolphin neutrophil NADPH-oxidase activation in a CpG motif-independent but phosphorothioate-dependent manner. (c) 2004 Elsevier Ltd. All rights reserved.

引用

页码：583 / 588

页数：6