Is BaF3 Bioassay Useful to Identify Patients with Bioinactive Growth Hormone?

被引：2

作者：

Pagani, Sara ^{[1
]}

Chaler, Eduardo A. ^{[2
]}

Meazza, Cristina ^{[1
]}

Maceiras, Mercedes ^{[2
]}

Gonzalez, Maria Eugenia ^{[2
]}

Rivarola, Marco A. ^{[2
]}

Cantoni, Francesca ^{[1
]}

Travaglino, Paola ^{[1
]}

Della Croce, Lucia ^{[3
]}

Laarej, Kamilia ^{[1
]}

Bozzola, Mauro ^{[1
]}

Belgorosky, Alicia ^{[2
]}

机构：

[1] Univ Pavia, Dept Pediat, I-27100 Pavia, Italy

[2] Garrahan Pediat Hosp, Endocrinol Lab, Buenos Aires, DF, Argentina

[3] Univ Pavia, Dept Math, I-27100 Pavia, Italy

来源：

JOURNAL OF PEDIATRIC ENDOCRINOLOGY & METABOLISM | 2010年 / 23卷 / 08期

关键词：

growth hormone; bioassay; gene mutation; cell line; GH biological activity; SHORT STATURE; GH DEFICIENCY; GENE MUTATION; HUMAN-SERUM; BIOACTIVITY; CHILDREN; ASSAY; FAILURE; SECRETION; TESTS;

D O I：

10.1515/jpem.2010.128

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We analyzed the ability of the BaF3 cell line bioassay to select patients with biologically inactive GH. We first evaluated the biological response of the Ba/F3-hGHR cells to rhGH additional doses from 10 to 5000 pg/ml. The concentration points corresponding to the linear part of the curve were selected. We then analyzed a group of sera, diluted like the standard, including the entire range of GH concentrations that can be analyzed by bioassay. The serum/standard area below the curve ratio was calculated. Serum GH immunoactivity determined by IMMULITE/GH bioactivity ratios was calculated. Our experimental data showed that GH-bioactivity/GH-immunoactivity ratios below 0.303 are indicative of a bioinactive GH molecule. This bioassay would recognize only extreme cases of GH bioinactivity, and it would not be a useful tool in the search for patients with altered forms of GH.

引用

页码：783 / 788

页数：6

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