Swollenin from Trichoderma reesei exhibits hydrolytic activity against cellulosic substrates with features of both endoglucanases and cellobiohydrolases

被引：62
作者：
Andberg, Martina [1 ]
Penttila, Merja [1 ]
Saloheimo, Markku [1 ]
机构：
[1] VTT Tech Res Ctr Finland, FI-02044 Espoo, Finland
来源：
BIORESOURCE TECHNOLOGY | 2015年 / 181卷
基金：
芬兰科学院;
关键词：
Lignocellulose; Swollenin; Hydrolysis; Endoglucanase; Cellobiohydrolase; PROTEIN; SACCHARIFICATION; CELLULASE; EXPANSINS; CLEAVAGE; BIOMASS;
D O I：
10.1016/j.biortech.2015.01.024
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
The cellulolytic and hemicellulolytic enzymes of Trichoderma reesei comprise one of the best characterised enzyme systems involved in lignocellulose degradation. In this paper, swollenin (SWOI), a protein recognised based on its sequence similarity with plant expansins, has been characterised. SWOI and its catalytic domain were subjected to analysis of their hydrolytic activity on different soluble carbohydrate polymers. By measuring the production of reducing ends, zymogram-, and viscosity analysis, SWOI was shown to have activity on substrates containing beta-1,4 glucosidic bonds, i.e. carboxymethyl cellulose, hydroxyethyl cellulose and beta-glucan. The formation of oligosaccharides from beta-glucan was analysed by HPLC and showed cellobiose as the main reaction product. SWOI was also able to hydrolyse soluble cello-oligosaccharides and the products formed were all consistent with SWOI cleaving a cellobiose unit off the substrate. In conclusion, the T. reesei swollenin showed a unique mode of action with similarities with action of both endoglucanases and cellobiohydrolases. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：105 / 113
页数：9
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