Further aspects on the hemolytic activity of the antibiotic lipopeptide iturin A

被引:120
作者
Aranda, FJ [1 ]
Teruel, JA [1 ]
Ortiz, A [1 ]
机构
[1] Univ Murcia, Fac Vet, Dept Bioquim & Biol Mol A, E-30100 Murcia, Spain
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2005年 / 1713卷 / 01期
关键词
iturin; lipopeptides; biosurfactants; liposomes; hemolysis;
D O I
10.1016/j.bbamem.2005.05.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The bacterial lipopeptide iturin A is able to cause hemolysis of human erythrocytes in a dose-dependent manner. Hemolysis takes place at iturin concentrations below its critical micellar concentration. Relative kinetics determinations clearly show that K+ leakage occurs prior to hemoglobin release. Furthermore, hemolysis can be prevented by addition to the outer solution of osmotic protectants of appropriate size. Altogether these results indicate that iturin A-induced hemolysis follows a colloid-osmotic mechanism, with the formation of a membrane pore of average diameter 32 angstrom. Iturin A is capable of inducing leakage of an aqueous fluorescent probe trapped in human erythrocyte ghosts, but not in large unilamellar liposomes made of various lipid compositions. The different permeabilizing effects of iturin A on model and biological membranes are discussed on the light of the presented results. (c) 2005 Elsevier B.V. All rights reserved.
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页码:51 / 56
页数:6
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