Application of gelatin zymography for evaluating low levels of contaminating neutrophils in red blood cell samples

被引：19

作者：

Achilli, Cesare ^{[1
]}

Ciana, Annarita ^{[1
]}

Balduini, Cesare ^{[1
]}

Risso, Angela ^{[2
]}

Minetti, Giampaolo ^{[1
]}

机构：

[1] Univ Pavia, Dept Biochem, I-27100 Pavia, Italy

[2] Univ Udine, Dept Biol & Plant Protect, I-33100 Udine, Italy

来源：

ANALYTICAL BIOCHEMISTRY | 2011年 / 409卷 / 02期

关键词：

D O I：

10.1016/j.ab.2010.10.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Supposedly "homogeneous" red blood cell (RBC) samples are commonly obtained by "washing" whole blood free of plasma, platelets, and white cells with physiological solutions, a procedure that does not result, however, in sufficient removal of polymorphonuclear neutrophils (PMNs), leading to possible arti-factual results. Pure RBC samples can be obtained only by leukodepletion procedures. Proposed here is a version of gelatin zymography adapted to detect matrix metalloproteinase 9 (MMP-9), selectively expressed by PMNs, in heterogeneous mixtures of RBCs and PMNs that can reveal contamination at levels as low as 1 PMN/10(6) RBCs. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：296 / 297

页数：2

共 8 条

[1] Neutrophil granulocytes uniquely express, among human blood cells, high levels of Methionine-sulfoxide-reductase enzymes [J].