Extraction optimization of bioactive compounds from ora-pro-nobis (Pereskia aculeata Miller) leaves and their in vitro antioxidant and antihemolytic activities

被引：31

作者：

Cruz, Thiago Mendanha ^{[1
]}

Santos, Janio Sousa ^{[2
]}

do Carmo, Mariana Araujo Vieira ^{[3
]}

Hellstrom, Jarkko ^{[4
]}

Pihlava, Juha-Matti ^{[4
]}

Azevedo, Luciana ^{[3
]}

Granato, Daniel ^{[2
,4
]}

Marques, Mariza Boscacci ^{[1
]}

机构：

[1] Univ Estadual Ponta Grossa, Grad Program Chem, Ave Carlos Cavalcanti,4748, BR-84030900 Ponta Grossa, PR, Brazil

[2] Univ Estadual Ponta Grossa, Grad Program Food Sci & Technol, Ave Carlos Cavalcanti,4748, BR-84030900 Ponta Grossa, PR, Brazil

[3] Univ Fed Alfenas, Nutr Fac, Rua Gabriel Monteiro da Silva,714, BR-37130000 Alfenas, MG, Brazil

[4] Nat Resources Inst Finland Luke, Food Proc & Qual, Prod Syst Unit, Latokartanonkaari 9, Helsinki 00790, Finland

来源：

FOOD CHEMISTRY | 2021年 / 361卷

关键词：

Non-conventional food plant; Hemolysis; Flavonols; Aldaric acid esters of phenolic acids; Reactive oxygen species; Response surface methodology; BIOLOGICAL-ACTIVITIES; FLAVONOIDS; PROTEINS; CAPACITY; STRESS;

D O I：

10.1016/j.foodchem.2021.130078

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Ora-pro-nobis (Pereskia aculeata Miller) is a non-conventional food plant common in Brazil. The objective of this study was to optimize the extraction of bioactive phenolic compounds from ora-pro-nobis leaves by employing solvent mixtures. Ten extracts were obtained with water, ethanol, acetone, and their binary and ternary mixtures, evaluating the chemical composition, antioxidant activity and bioactivities in vitro. The response surface methodology was applied to model the results and calculate the optimal solvent composition, which is 60% water, 40% ethanol and 0% acetone. The optimized extract is rich in phenolic compounds (64 mg GAE/g) and proteins (823 mg/g) and presents antioxidant activity (in intracellular media as well) and inhibits lipid peroxidation (32%) along with hypotonic hemolysis (H50 = 0.339%), it does not present toxicity in vitro against cancer and normal cells. This is the first report of chicoric, caffeoyl-hexaric and coumaroyl-hexaric acids and some glycosylate derivatives of flavonols in ora-pro-nobis leaves.

引用

页数：12

共 39 条

[1] Pereskia aculeata Miller leaves present in vivo topical anti-inflammatory activity in models of acute and chronic dermatitis [J].