In vitro fertilization with cryopreserved inbred mouse sperm

被引：154

作者：

Sztein, JM ^{[1
]}

Farley, JS ^{[1
]}

Mobraaten, LE ^{[1
]}

机构：

[1] Jackson Lab, Bar Harbor, ME 04609 USA

来源：

BIOLOGY OF REPRODUCTION | 2000年 / 63卷 / 06期

关键词：

fertilization; IVF/ART; reproductive behavior;

D O I：

10.1095/biolreprod63.6.1774

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sperm from C57BL/6J, DBA/2J, BALB/cJ, 129S3/SvImJ, and FVB/NJ inbred mice were cryopreserved in 3% skim milk/18% raffinose cryoprotectant solution. The post-thaw sperm from ail strains were evaluated for their viability and fertility by comparing them against B6D2F1 sperm used as a control. The protocol used for freezing mouse sperm was effective in different strains, because the motility was decreased by 50% after cryopreservation similar to other mammalian sperm. However, the progressive motility and the fertility of each inbred strain were affected differently. The C57BL/6J, BALB/cJ, and 129S3/SvImJ strains were the most affected; their fertility (two-cell cleavage) decreased from 70%, 34%, and 84% when using freshly collected sperm to 6%, 12%, and 6% when using frozen/thawed sperm, respectively. Live newborns derived from frozen/thawed sperm were obtained from all strains in the study. These results corroborate the genetic variation among strains with regard to fertility and susceptibility to cryopreservation.

引用

页码：1774 / 1780

页数：7

共 51 条

[1] Normal mice develop from oocytes injected with spermatozoa with grossly misshapen heads [J].