fracture;
bone repair;
immobilization;
osteoporosis;
mouse;
D O I:
10.1016/j.bone.2004.09.010
中图分类号:
R5 [内科学];
学科分类号:
1002 ;
100201 ;
摘要:
The present study was carried out to determine whether immobilization-induced (Im) osteopenic bone possesses the same reparative capacity as normal healthy bone. Furthermore, the effects of mechanical loading versus immobilization on bone defect healing were studied. Three-week cast-immobilization was used to induce local osteopenia in mice. A standardized metaphyseal bone defect of the distal femur was created unilaterally both in immobilization-induced (Im) osteopenic mice and in nonimmobilized (Mo) age-matched control animals. After creation of the bone defect, the animals in both groups were further divided into two groups: 3-week cast-immobilization (Im-Im and Mo-Im) groups, and unrestricted weight-bearing (Im-Mo and Mo-Mo) groups. The healing process was followed up to 3 weeks using RNA analysis, histomorphometry, biomechanical testing, and pQCT measurements. At 3 weeks of healing without immobilization, bone mineral density (BMD), as well as bone bending stiffness and strength were higher in normal (Mo-Mo) than in osteopenic (Im-Mo) bone. Although the levels of mRNAs characteristic to chondrocytes (Sox9 and type II collagen), hypertrophic chondrocytes (Type X collagen), osteoblasts (type I collagen and osteocalcin), and osteoclasts (cathepsin K) during the bone defect healing exhibited similarities in their expression profiles, mechanical loading conditions also caused characteristic differences. Mechanical loading during healing (Mo-Mo group) induced stronger expression of cartilage- and bone-specific genes and resulted in higher BMD than that seen in the cast-immobilized group (Mo-Im). In biomechanical analysis, increased bending stiffness and strength were also observed in animals that were allowed weight-bearing during healing. Thus, our study shows that bone healing follows the same molecular pathway both in osteopenic and normal bones and presents evidence for reduced or delayed regeneration of noncritical size defects in immobilization-induced osteopenic bone. (C) 2004 Elsevier Inc. All rights reserved.
机构:
Ryerson Univ, Dept Mech & Ind Engn, Toronto, ON, CanadaRyerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada
Coquim, Jason
Clemenzi, Joseph
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机构:
Ryerson Univ, Dept Mech & Ind Engn, Toronto, ON, CanadaRyerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada
Clemenzi, Joseph
Salahi, Mohsen
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h-index: 0
机构:
Ryerson Univ, Dept Mech & Ind Engn, Toronto, ON, CanadaRyerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada
Salahi, Mohsen
Sherif, Abdurahman
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Ryerson Univ, Dept Mech & Ind Engn, Toronto, ON, CanadaRyerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada
Sherif, Abdurahman
Avval, Pouria Tavakkoli
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机构:
Centennial Coll, Sch Engn Technol & Applied Sci, Toronto, ON, CanadaRyerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada
Avval, Pouria Tavakkoli
Shah, Suraj
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机构:
Ryerson Univ, Off Res Adm, Toronto, ON, CanadaRyerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada
Shah, Suraj
Schemitsch, Emil H.
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机构:
Victoria Hosp, Orthopaed Biomech Lab, London, ON, Canada
Western Univ, Dept Surg, London, ON, CanadaRyerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada
Schemitsch, Emil H.
Bagheri, Z. Shaghayegh
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机构:
UOIT, Dept Automot Mech & Mfg Engn, North Oshawa, ON, CanadaRyerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada
Bagheri, Z. Shaghayegh
Bougherara, Habiba
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机构:
Ryerson Univ, Dept Mech & Ind Engn, Toronto, ON, CanadaRyerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada
Bougherara, Habiba
Zdero, Radovan
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h-index: 0
机构:
Ryerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada
Victoria Hosp, Orthopaed Biomech Lab, London, ON, Canada
Western Univ, Dept Surg, London, ON, Canada
Western Univ, Dept Mech & Mat Engn, London, ON, CanadaRyerson Univ, Dept Mech & Ind Engn, Toronto, ON, Canada
机构:
Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Stomatol & Physiol, BR-05508 Sao Paulo, BrazilUniv Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Stomatol & Physiol, BR-05508 Sao Paulo, Brazil
Prado, KFB
Carvalho, TLL
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机构:
Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Stomatol & Physiol, BR-05508 Sao Paulo, BrazilUniv Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Stomatol & Physiol, BR-05508 Sao Paulo, Brazil
Carvalho, TLL
Franci, JAA
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h-index: 0
机构:
Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Stomatol & Physiol, BR-05508 Sao Paulo, BrazilUniv Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Stomatol & Physiol, BR-05508 Sao Paulo, Brazil
Franci, JAA
Brentegani, LG
论文数: 0引用数: 0
h-index: 0
机构:
Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Stomatol & Physiol, BR-05508 Sao Paulo, BrazilUniv Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Stomatol & Physiol, BR-05508 Sao Paulo, Brazil