Human Epidural AD-MSC Exosomes Improve Function Recovery after Spinal Cord Injury in Rats

被引：37

作者：

Sung, Soo-Eun ^{[1
,2
]}

Seo, Min-Soo ^{[1
]}

Kim, Young-In ^{[3
]}

Kang, Kyung-Ku ^{[1
]}

Choi, Joo-Hee ^{[1
]}

Lee, Sijoon ^{[1
]}

Sung, Minkyoung ^{[1
]}

Yim, Sang-Gu ^{[2
]}

Lim, Ju-Hyeon ^{[4
,5
]}

Seok, Hyun-Gyu ^{[4
]}

Yang, Seung-Yun ^{[2
]}

Lee, Gun-Woo ^{[3
,4
]}

机构：

[1] Daegu Gyeongbuk Med Innovat Fdn, Preclin Res Ctr, Daegu 41061, South Korea

[2] Pusan Natl Univ, Dept Biomat Sci, BK21 Four Program, Miryang 50463, South Korea

[3] Cellexobio Ltd, Daegu 42415, South Korea

[4] Yeungnam Univ, Med Ctr, Dept Orthoped Surg, Coll Med, 170 Hyochung Ro, Daegu 42415, South Korea

[5] Co Culture Team, Kolmar Korea Holdings 61,Heolleung Ro 8 Gill, Seoul 06800, South Korea

来源：

BIOMEDICINES | 2022年 / 10卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

spinal cord injury; mesenchymal stem cells; exosomes; extracellular vesicles; MESENCHYMAL STEM-CELL; MODELS; INFLAMMATION; EXPRESSION; PROGRESS; BCL3;

D O I：

10.3390/biomedicines10030678

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spinal cord injury (SCI) interferes with the normal function of the autonomic nervous system by blocking circuits between the sensory and motor nerves. Although many studies focus on functional recovery after neurological injury, effective neuroregeneration is still being explored. Recently, extracellular vesicles such as exosomes have emerged as cell-free therapeutic agents owing to their ability of cell-to-cell communication. In particular, exosomes released from mesenchymal stem cells (MSCs) have the potential for tissue regeneration and exhibit therapeutic effectiveness in neurological disorders. In this study, we isolated exosomes from human epidural adipose tissue-derived MSCs (hEpi AD-MSCs) using the tangential flow filtration method. The isolated exosomes were analyzed for size, concentration, shape, and major surface markers using nanoparticle tracking analysis, transmission electron microscopy, and flow cytometry. To evaluate their effect on SCI recovery, hEpi AD-MSC exosomes were injected intravenously in SCI-induced rats. hEpi AD-MSC exosomes improved the locomotor function of SCI-induced rats. The results of histopathological and cytokine assays showed that hEpi AD-MSC exosomes regulated inflammatory response. Genetic profiling of the rat spinal cord tissues revealed changes in the expression of inflammation-related genes after exosome administration. Collectively, hEpi AD-MSC exosomes are effective in restoring spinal functions by reducing the inflammatory response.

引用

页数：16

共 66 条

[1] Experimental spinal cord trauma: a review of mechanically induced spinal cord injury in rat models [J].