Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases

被引:24
作者
Li, Junwei [1 ,2 ]
Wang, Yuexia [2 ]
Wang, Bin [2 ]
Lou, Juan [2 ]
Ni, Peng [2 ]
Jin, Yuefei [2 ]
Chen, Shuaiyin [2 ]
Duan, Guangcai [2 ]
Zhang, Rongguang [1 ,2 ]
机构
[1] Hainan Med Univ, Int Sch Publ Hlth & One Hlth, Affiliated Hosp 1, Haikou 570102, Hainan, Peoples R China
[2] Zhengzhou Univ, Coll Publ Hlth, Dept Epidemiol, Zhengzhou 450001, Peoples R China
基金
中国国家自然科学基金;
关键词
CRISPR; Cas system; biosensing technologies; pathogen nucleic acids; SARS-CoV-2; diagnoses; RNA-GUIDED ENDONUCLEASE; CAS SYSTEMS; ISOTHERMAL AMPLIFICATION; ADAPTIVE IMMUNITY; CRISPR-CAS9; DNA; CLEAVAGE; COMPLEX; BACTERIA; PLATFORM;
D O I
10.3390/diagnostics12102455
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The CRISPR/Cas system is a protective adaptive immune system against attacks from foreign mobile genetic elements. Since the discovery of the excellent target-specific sequence recognition ability of the CRISPR/Cas system, the CRISPR/Cas system has shown excellent performance in the development of pathogen nucleic-acid-detection technology. In combination with various biosensing technologies, researchers have made many rapid, convenient, and feasible innovations in pathogen nucleic-acid-detection technology. With an in-depth understanding and development of the CRISPR/Cas system, it is no longer limited to CRISPR/Cas9, CRISPR/Cas12, and other systems that had been widely used in the past; other CRISPR/Cas families are designed for nucleic acid detection. We summarized the application of CRISPR/Cas-related technology in infectious-disease detection and its development in SARS-CoV-2 detection.
引用
收藏
页数:24
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