Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases

被引：24

作者：

Li, Junwei ^{[1
,2
]}

Wang, Yuexia ^{[2
]}

Wang, Bin ^{[2
]}

Lou, Juan ^{[2
]}

Ni, Peng ^{[2
]}

Jin, Yuefei ^{[2
]}

Chen, Shuaiyin ^{[2
]}

Duan, Guangcai ^{[2
]}

Zhang, Rongguang ^{[1
,2
]}

机构：

[1] Hainan Med Univ, Int Sch Publ Hlth & One Hlth, Affiliated Hosp 1, Haikou 570102, Hainan, Peoples R China

[2] Zhengzhou Univ, Coll Publ Hlth, Dept Epidemiol, Zhengzhou 450001, Peoples R China

来源：

DIAGNOSTICS | 2022年 / 12卷 / 10期

基金：

中国国家自然科学基金;

关键词：

CRISPR; Cas system; biosensing technologies; pathogen nucleic acids; SARS-CoV-2; diagnoses; RNA-GUIDED ENDONUCLEASE; CAS SYSTEMS; ISOTHERMAL AMPLIFICATION; ADAPTIVE IMMUNITY; CRISPR-CAS9; DNA; CLEAVAGE; COMPLEX; BACTERIA; PLATFORM;

D O I：

10.3390/diagnostics12102455

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The CRISPR/Cas system is a protective adaptive immune system against attacks from foreign mobile genetic elements. Since the discovery of the excellent target-specific sequence recognition ability of the CRISPR/Cas system, the CRISPR/Cas system has shown excellent performance in the development of pathogen nucleic-acid-detection technology. In combination with various biosensing technologies, researchers have made many rapid, convenient, and feasible innovations in pathogen nucleic-acid-detection technology. With an in-depth understanding and development of the CRISPR/Cas system, it is no longer limited to CRISPR/Cas9, CRISPR/Cas12, and other systems that had been widely used in the past; other CRISPR/Cas families are designed for nucleic acid detection. We summarized the application of CRISPR/Cas-related technology in infectious-disease detection and its development in SARS-CoV-2 detection.

引用

页数：24

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