Functional domains of tat required for efficient human immunodeficiency virus type 1 reverse transcription

被引:40
|
作者
Ulich, C
Dunne, A
Parry, E
Hooker, CW
Gaynor, RB
Harrich, D
机构
[1] Royal Childrens Hosp, Sir Albert Sakzewski Virus Res Ctr, Natl Ctr HIV Virol Res, HIV Res Unit, Herston, Qld 4029, Australia
[2] Univ Texas, SW Med Ctr, Dept Internal Med, Div Hematol & Oncol, Dallas, TX 75235 USA
[3] Univ Texas, SW Med Ctr, Dept Microbiol, Dallas, TX 75235 USA
关键词
D O I
10.1128/JVI.73.3.2499-2508.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Tat expression is required for efficient human immunodeficiency virus type 1 (HIV-1) reverse transcription. In the present study, we generated a series of 293 cell lines that contained a provirus with a tat gene deletion (Delta tat). Cell lines that contained Delta tat and stably transfected vectors containing either wild-type tat or a number of tat mutants were obtained so that the abilities of these tat genes to stimulate HIV-1 gene expression and reverse transcription could be compared. tat genes with mutations in the amino terminus did not stimulate either viral gene expression or HIV-1 reverse transcription. In contrast, tat mutants in the activation, core, and basic domains of Tat did not stimulate HIV-1 gene expression but markedly stimulated HIV-1 reverse transcription. No differences in the levels of virion genomic RNA or tRNA(3)(Lys) were seen in the HIV-1 Delta tat viruses complemented with either mutant or wild type tat. Finally, overexpression of the Tat-associated kinases CDK7 and CDK9, which are involved in Tat activation of HIV-1 transcription, was not able to complement the reverse transcription defects associated with the lack of a functional tat gene. These results indicate that the mechanism by which tat modulates HIV-1 reverse transcription is distinct from its ability to activate HIV-1 gene expression.
引用
收藏
页码:2499 / 2508
页数:10
相关论文
共 50 条
  • [1] Human immunodeficiency virus type 1 protease regulation of tat activity is essential for efficient reverse transcription and replication
    Apolloni, A
    Hooker, CW
    Mak, J
    Harrich, D
    JOURNAL OF VIROLOGY, 2003, 77 (18) : 9912 - 9921
  • [2] Human immunodeficiency virus type 1 reverse transcription is stimulated by Tat from other lentiviruses
    Hooker, CW
    Scott, J
    Apolloni, A
    Parry, E
    Harrich, D
    VIROLOGY, 2002, 300 (02) : 226 - 235
  • [3] Tat is required for efficient HIV-1 reverse transcription
    Harrich, D
    Ulich, C
    GarciaMartinez, LF
    Gaynor, RB
    EMBO JOURNAL, 1997, 16 (06): : 1224 - 1235
  • [4] Role of Vif in human immunodeficiency virus type 1 reverse transcription
    Goncalves, J
    Korin, Y
    Zack, J
    Gabuzda, D
    JOURNAL OF VIROLOGY, 1996, 70 (12) : 8701 - 8709
  • [5] Biochemical and functional interaction of the human immunodeficiency virus type 1 Tat transactivator with the general transcription factor TFIIB
    Veschambre, P
    Roisin, A
    Jalinot, P
    JOURNAL OF GENERAL VIROLOGY, 1997, 78 : 2235 - 2245
  • [6] A critical role for the TAR element in promoting efficient human immunodeficiency virus type 1 reverse transcription
    Harrich, D
    Ulich, C
    Gaynor, RB
    JOURNAL OF VIROLOGY, 1996, 70 (06) : 4017 - 4027
  • [7] Targeting Tat inhibitors in the assembly of human immunodeficiency virus type 1 transcription complexes
    D'Orso, Ivan
    Grunwell, Jocelyn R.
    Nakamura, Robert L.
    Das, Chandreyee
    Frankel, Alan D.
    JOURNAL OF VIROLOGY, 2008, 82 (19) : 9492 - 9504
  • [8] JUXTAPOSITION BETWEEN ACTIVATION AND BASIC DOMAINS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TAT IS REQUIRED FOR OPTIMAL INTERACTIONS BETWEEN TAT AND TAR
    LUO, Y
    PETERLIN, BM
    JOURNAL OF VIROLOGY, 1993, 67 (06) : 3441 - 3445
  • [9] The TAR hairpin of human immunodeficiency virus type 1 can be deleted when not required for Tat-mediated activation of transcription
    Das, Atze T.
    Harwig, Alex
    Vrolijk, Martine M.
    Berkhout, Ben
    JOURNAL OF VIROLOGY, 2007, 81 (14) : 7742 - 7748
  • [10] Characterization of intracellular reverse transcription complexes of human immunodeficiency virus type 1
    Fassati, A
    Goff, SP
    JOURNAL OF VIROLOGY, 2001, 75 (08) : 3626 - 3635