Towards an Electrochemical Immunosensor for the Detection of Antibodies against SARS-CoV-2 Spike Protein

被引：48
作者：
Liustrovaite, Viktorija [1 ]
Drobysh, Maryia [1 ,2 ]
Rucinskiene, Alma [1 ,2 ]
Baradoke, Ausra [2 ]
Ramanaviciene, Almira [1 ]
Plikusiene, Ieva [1 ,2 ]
Samukaite-Bubniene, Urte [1 ]
Viter, Roman [3 ]
Chen, Chien-Fu [2 ]
Ramanavicius, Arunas [1 ,2 ]
机构：
[1] Vilnius Univ, Fac Chem & Geosci, NanoTechnas Ctr Nanotechnol & Mat Sci, Naugarduko Str 24, LT-03225 Vilnius, Lithuania
[2] State Res Inst, Ctr Phys & Technol Sci, Sauletekio Ave 2, Vilnius, Lithuania
[3] Univ Latvia, Inst Atom Phys & Spect, Jelgavas St 3, LV-1004 Riga, Latvia
来源：
JOURNAL OF THE ELECTROCHEMICAL SOCIETY | 2022年 / 169卷 / 03期
关键词：
COVID-19; SARS-CoV-2; Electrochemical immunosensor; Electrochemical impedance spectroscopy; Cyclic voltammetry; Self-assembled monolayer (SAM); Antigen-antibody complex; SELF-ASSEMBLED MONOLAYERS; GOLD;
D O I：
10.1149/1945-7111/ac5d91
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
Herein we report the electrochemical system for the detection of specific antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins in blood serum patient samples after coronavirus disease 2019 (COVID-19). For this purpose, the recombinant SARS-CoV-2 spike protein (SCoV2-rS) was covalently immobilised on the surface of the gold electrode pre-modified with mixed self-assembled monolayer (SAMmix) consisting of 11-mercaptoundecanoic acid and 6-mercapto-1-hexanol. The affinity interaction of SCoV2-rS with specific antibodies against this protein (anti-rS) was detected using two electrochemical methods: cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The anti-rS was detected with a detection limit of 2.53 nM and 1.99 nM using CV and EIS methods, respectively. The developed electrochemical immunosensor is suitable for the confirmation of COVID-19 infection or immune response in humans after vaccination.
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页数：7
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