Kinetic Capillary Electrophoresis with Mass-Spectrometry Detection (KCE-MS) Facilitates Label-Free Solution-Based Kinetic Analysis of Protein-Small Molecule Binding

被引：16

作者：

Bao, Jiayin ^{[1
,2
]}

Krylova, Svetlana M. ^{[1
,2
]}

Wilson, Derek J. ^{[1
,2
]}

Reinstein, Oren ^{[1
,2
]}

Johnson, Philip E. ^{[1
,2
]}

Krylov, Sergey N. ^{[1
,2
]}

机构：

[1] York Univ, Dept Chem, Toronto, ON M3J 1P3, Canada

[2] York Univ, Ctr Res Biomol Interact, Toronto, ON M3J 1P3, Canada

来源：

CHEMBIOCHEM | 2011年 / 12卷 / 17期

基金：

加拿大自然科学与工程研究理事会;

关键词：

electrophoresis; label-free kinetics assays; mass spectrometry; proteins; small molecules; EQUILIBRIUM MIXTURES; LIGAND-BINDING; H/D EXCHANGE; TITRATION; CONSTANTS;

D O I：

10.1002/cbic.201100617

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：2551 / 2554

页数：4

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