Architecting of an aptasensor for the staphylococcus aureus analysis by modification of the screen-printed carbon electrode with aptamer/Ag-Cs-Gr QDs/NTiO2

被引：43

作者：

Ghalkhani, Masoumeh ^{[1
]}

Sohouli, Esmail ^{[1
]}

Khaloo, Shokooh Sadat ^{[2
,3
]}

Vaziri, Mohammad Hossein ^{[2
,3
]}

机构：

[1] Shahid Rajaee Teacher Training Univ, Fac Sci, Dept Chem, Electrochem Sensors Res Lab, POB 1678815811, Tehran, Iran

[2] Shahid Beheshti Univ Med Sci, Workpl Hlth Promot Res Ctr, Tehran, Iran

[3] Shahid Beheshti Univ Med Sci, Sch Publ Hlth & Safety, Dept Hlth Safety & Environm, Tehran, Iran

来源：

CHEMOSPHERE | 2022年 / 293卷

关键词：

Staphylococcus aureus; Aptamer; Electrochemical; Graphene quantom dot; Silver nanoparticles; REDUCED GRAPHENE OXIDE; SENSITIVE DETECTION; QUANTUM DOTS; NANOCOMPOSITE; BIOSENSOR; BACTERIA; GENE; IDENTIFICATION; NANOPARTICLES; FABRICATION;

D O I：

10.1016/j.chemosphere.2022.133597

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Given the many issues bacterial infections cause to humans and the necessity for their detection, in this work we developed a robust aptasensor for prompt, ultrasensitive, and selective analysis of staphylococcus aureus bacterium (S. aureus). A nanocomposite of Ag nanoparticles, chitosan, graphene quantum dots, and nitrogen-doped TiO2 nanoparticles (Ag-Cs-Gr QDs/NTiO2) was synthesized, and thoroughly characterized by XRD, FT-IR, and FE-SEM spectroscopic methods. The surface of screen-printed carbon electrodes modified with Ag-Cs-Gr QDs/ NTiO2 nanocomposite was utilized as a compatible platform for aptamer attachment. The aptasensor accurately determined S. aureus in the dynamic range of 10(-5) x 10(8) CFU/mL with detection limit of 3.3 CFU/mL. The monitoring of the practical performance of aptasensor in human serum samples revealed its superiority over the conventional methods (relative recovery of 96.25-103.33%). The Ag-Cs-Gr QDs/NTiO2-based aptasensor offers facile, biocompatibility, good repeatability, reproducibility (RSD = 3.66%), label free and stabile strategy for sensitive S. aureus analysis free from biomolecules interferences in actual specimens.

引用

页数：10