Muscarinic acetylcholine receptor-mediated increase of angiotensin type 2 receptor mRNA in PC12 cells

被引：7

作者：

Shibata, K ^{[1
]}

Ikuko, W

Inoue, K

Katsuragi, T

机构：

[1] Fukuoka Univ, Sch Med, Res Lab Biodynam, Fukuoka 8140181, Japan

[2] Fukuoka Univ, Sch Med, Dept Pharmacol, Fukuoka 8140181, Japan

[3] Natl Inst Hlth Sci, Div Pharmacol, Tokyo 158, Japan

来源：

NEUROREPORT | 1998年 / 9卷 / 17期

关键词：

angiotensin type 2 receptor; carbachol; nitric oxide; PC12; cells; protein kinase C;

D O I：

10.1097/00001756-199812010-00004

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE present study examined changes in the angiotensin type 2 (AT2) receptor mRNA level after carbachol exposure in PC12 cells. The AT2 receptor mRNA level increased 2- to-3-fold after 12-18 h of carbachol exposure (100 mu M) The up-regulation of AT2 receptor mRNA was antagonized by atropine, which is a muscarinic receptor antagonist, thus suggesting that the increase in AT2 receptor mRNA is mediated via muscarinic acetylcholine receptors. This increase is blocked by N-G-nitro-L-arginine-methylester, nitric oxide (NO) synthase inhibitor, and hemoglobin NO trap. Protein kinase C (PKC) inhibitors, H-7 and calphostin C, inhibited the. carbachol-induced upregulation. In addition, a G kinase inhibitor, ODQ (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one), inhibited this increase. These findings suggest that the carbachol-induced increases in AT2 receptor mRNA is regulated by the activation of NO-cGMP and/or the PKC pathway. NeuroReport 9: 3783-3789 (C) 1998 Lippincott Williams & Wilkins.

引用

页码：3783 / 3789

页数：7

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