Evaluation of the eazyplex® SuperBug CRE system for rapid detection of carbapenemases and ESBLs in clinical Enterobacteriaceae isolates recovered at two Spanish hospitals

被引:47
作者
Garcia-Fernandez, Sergio [1 ,2 ]
Morosini, Maria-Isabel [1 ,2 ,3 ]
Marco, Francesc [4 ,5 ]
Gijon, Desiree [1 ,2 ,3 ]
Vergara, Andrea [4 ,5 ]
Vila, Jordi [4 ,5 ]
Ruiz-Garbajosa, Patricia [1 ,2 ,3 ]
Canton, Rafael [1 ,2 ,3 ]
机构
[1] Hosp Univ Ramon y Cajal, Serv Microbiol, Madrid, Spain
[2] IRYCIS, Madrid, Spain
[3] Red Espanola Invest Patol Infecciosa, Madrid, Spain
[4] Univ Barcelona, Sch Med, Dept Clin Microbiol, CDB,Hosp Clin, Barcelona, Spain
[5] Barcelona Ctr Int Hlth Res CRESIB, Barcelona, Spain
关键词
isothermal amplification; beta-lactamases; LAMP; ESCHERICHIA-COLI STRAIN; KLEBSIELLA-PNEUMONIAE; BETA-LACTAMASE; IDENTIFICATION; PRODUCERS; EMERGENCE; SPREAD; GENES;
D O I
10.1093/jac/dku476
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: To evaluate the performance of the eazyple (R) SuperBug CRE system, a loop-mediated isothermal amplification (LAMP)-based system, for confirming the presence of carbapenemases in addition to CTX-M-type ESBLs in previously genotypically and/or phenotypically characterized clinical Enterobacteriaceae isolates recovered in two centres in Spain. Methods: A collection of 94 carbapenemase-producing strains previously characterized by conventional PCR and sequencing and a total of 45 prospectively collected isolates with phenotypes compatible with the presence of a carbapenemase were tested with the eazyple (R) SuperBug CRE system. In both cases, the presence of an ESBL was also assessed. Results were evaluated to establish the accuracy of this rapid LAMP-based system aswell as to determine the concordance between all approaches. Results: The eazyple (R) SuperBug CRE system correctly detected bla carbapenemase genes with or without blaCTX-M genes in 100% of the molecularly characterized strains. Absolute concordance (100%) was also observed in the case of isolates with phenotypes compatible with the presence of a carbapenemase with or without an ESBL inferred by susceptibility patterns and phenotypic inhibitory profiles. Determinations performed with the eazyple (R) SuperBug CRE system took 15 min. Conclusions: The eazyple (R) SuperBug CRE system proved to be a powerful tool for the detection of different carbapenemases as well as CTX-M-type ESBLs in Enterobacteriaceae with a rapid resolution time. The test has the high-performance parameters attributable to the sensitivity and specificity already demonstrated by LAMP-based assays. These results assure the usefulness of this test for routine rapid confirmation of carbapenemase-producing Enterobacteriaceae.
引用
收藏
页码:1047 / 1050
页数:4
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