Use of fluorescent Ca2+ dyes with green fluorescent protein and its variants:: problems and solutions

被引：36

作者：

Bolsover, S ^{[1
]}

Ibrahim, O ^{[1
]}

O'Luanaigh, N ^{[1
]}

Williams, H ^{[1
]}

Cockcroft, S ^{[1
]}

机构：

[1] UCL, Dept Physiol, London WC1E 6BT, England

来源：

BIOCHEMICAL JOURNAL | 2001年 / 356卷 / 02期

关键词：

blue fluorescent protein; cross-talk; fura; 2; green fluorescent protein; X-Rhod; 1;

D O I：

10.1042/0264-6021:3560345

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the degree to which fluorescent Ca2+ indicator dyes, and green fluorescent protein and its variants, can be used together. We find that the most commonly used fluorescent protein, enhanced green fluorescent protein (EGFP), seriously contaminates fura 2 signals. We suggest two alternative combinations for which there is no detectable contamination of the Ca2+ indicator signal by the fluorescent protein. Blue fluorescent protein can be used with the Ca2+ indicator Fura Red, EGFP can be used with the Ca2+ indicator X-Rhod 1. The use of these combinations will permit the accurate measurement of Ca2+ signals in cells transfected with fluorescent proteins.

引用

页码：345 / 352

页数：8

共 13 条

[1] Acute overexpression of lactate dehydrogenase-A perturbs β-cell mitochondrial metabolism and insulin secretion [J].