A H2O2 biosensor based on immobilization of horseradish peroxidase in a gelatine network matrix

被引：8
|
作者：
Yao, H [1 ]
Li, N [1 ]
Wei, YL [1 ]
Zhu, JJ [1 ]
机构：
[1] Nanjing Univ, Dept Chem, Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China
来源：
SENSORS | 2005年 / 5卷 / 4-5期
关键词：
H2O2; biosensor; horseradish peroxidase; gelatine matrix;
D O I：
10.3390/s5040277
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple and promising H2O2 biosensor has been developed by successful entrapment of horseradish peroxidase (HRP) in a gelatine matrix which was cross-linked with formaldehyde. The large microscopic surface area and porous morphology of the gelatine matrix lead to high enzyme loading and the enzyme entrapped in this matrix can retain its bioactivity. This biosensor exhibited a fast amperometric response to hydrogen peroxide (H2O2). The linear range for H2O2 determination was from 2.5 x 10(-5) to 2.5 x 10(-3) M, with a detection limit of 2.0 x 10(-6) M based on S/N = 3. This biosensor possessed very good reproducibility.
引用
收藏
页码：277 / 283
页数：7
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