Pulsed laser deposition of pepsin thin films

被引：10

作者：

Kecskeméti, G

Kresz, N

Smausz, T

Hopp, B

Nógrádi, A

机构：

[1] Univ Szeged, Dept Opt & Quantum Elect, H-6720 Szeged, Hungary

[2] Hungarian Acad Sci, H-6720 Szeged, Hungary

[3] Univ Szeged, Res Grp Laser Phys, H-6720 Szeged, Hungary

[4] Univ Szeged, Dept Ophthalmol, H-6720 Szeged, Hungary

来源：

APPLIED SURFACE SCIENCE | 2005年 / 247卷 / 1-4期

基金：

匈牙利科学研究基金会;

关键词：

pulsed laser deposition; thin films; pepsin; FTIR;

D O I：

10.1016/j.apsusc.2005.01.144

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Pulsed laser deposition (PLD) of organic and biological thin films has been extensively studied due to its importance in medical applications among others. Our investigations and results on PLD of a digestion catalyzing enzyme, pepsin, are presented. Targets pressed from pepsin powder were ablated with pulses of an ArF excimer laser (lambda = 193 nm, FWHM = 30 ns), the applied fluence was varied between 0.24 and 5.1 J/cm(2). The pressure in the PLD chamber was 2.7 x 10(-3) Pa. The thin layers were deposited onto glass and KBr substrates. Our IR spectroscopic measurements proved that the chemical composition of deposited thin films is similar to that of the target material deposited at 0.5 and 1.3 J/cm(2). The protein digesting capacity of the transferred pepsin was tested by adapting a modified "protein cube" method. Dissolution of the ovalbumin sections proved that the deposited layers consisted of catalytically active pepsin. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：83 / 88

页数：6

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