Characterization of Endothelial and Smooth Muscle Cells From Different Canine Vessels

被引:21
作者
Oosterhoff, Loes A. [1 ]
Kruitwagen, Hedwig S. [1 ]
van Wolferen, Monique E. [1 ]
van Balkom, Bas W. M. [2 ]
Mokry, Michal [3 ,4 ]
Lansu, Nico [3 ,4 ]
van den Dungen, Noortje A. M. [4 ,5 ]
Penning, Louis C. [1 ]
Spanjersberg, Talitha C. F. [1 ]
de Graaf, Johannes W. [1 ]
Veenendaal, Tomas [1 ]
Zomerdijk, Flin [1 ]
Fledderus, Joost O. [2 ]
Spee, Bart [1 ]
van Steenbeek, Frank G. [1 ]
机构
[1] Univ Utrecht, Fac Vet Med, Dept Clin Sci Compan Anim, Utrecht, Netherlands
[2] Univ Med Ctr Utrecht, Div Internal Med & Dermatol, Nephrol & Hypertens, Utrecht, Netherlands
[3] Univ Med Ctr Utrecht, Wilhelmina Childrens Hosp, Div Pediat, Utrecht, Netherlands
[4] Univ Med Ctr Utrecht, Epigen Facil, Utrecht, Netherlands
[5] Univ Med Ctr Utrecht, Div Heart & Lungs, Dept Cardiol, Utrecht, Netherlands
关键词
angiogenesis; cell model system; endothelial cells; vascular cell interaction; vascular smooth muscle cells; STEM-CELLS; DUCTUS-ARTERIOSUS; IN-VIVO; ANGIOGENESIS; EXPRESSION; COCULTURE; FIBROBLASTS; MODEL; TOOL; RNA;
D O I
10.3389/fphys.2019.00101
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Vasculature performs a critical function in tissue homeostasis, supply of oxygen and nutrients, and the removal of metabolic waste products. Vascular problems are implicated in a large variety of pathologies and accurate in vitro models resembling native vasculature are of great importance. Unfortunately, existing in vitro models do not sufficiently reflect their in vivo counterpart. The complexity of vasculature requires the examination of multiple cell types including endothelial cells (ECs) and vascular smooth muscle cells (VSMCs), as well as vessel location in the body from which they originate. The use of canine blood vessels provides a way to study vasculature with similar vessel size and physiology compared to human vasculature. We report an isolation procedure that provides the possibility to isolate both the endothelial and smooth muscle cells from the same vessels simultaneously, enabling new opportunities in investigating vasculature behavior. Canine primary ECs and VSMCs were isolated from the vena cava, vena porta and aorta. All tissue sources were derived from three donors for accurate comparison and to reduce inter-animal variation. The isolation and purification of the two distinct cell types was confirmed by morphology, gene- and protein-expression and function. As both cell types can be derived from the same vessel, this approach allows accurate modeling of vascular diseases and can also be used more widely, for example, in vascular bioreactors and tissue engineering designs. Additionally, we identified several new genes that were highly expressed in canine ECs, which may become candidate genes for novel EC markers. In addition, we observed transcriptional and functional differences between arterial- and venous-derived endothelium. Further exploration of the transcriptome and physiology of arteriovenous differentiation of primary cells may have important implications for a better understanding of the fundamental behavior of the vasculature and pathogenesis of vascular disease.
引用
收藏
页数:11
相关论文
共 51 条
[41]   OXYGEN RADICALS MEDIATE ENDOTHELIAL CELL DAMAGE BY COMPLEMENT-STIMULATED GRANULOCYTES - INVITRO MODEL OF IMMUNE VASCULAR DAMAGE [J].
SACKS, T ;
MOLDOW, CF ;
CRADDOCK, PR ;
BOWERS, TK ;
JACOB, HS .
JOURNAL OF CLINICAL INVESTIGATION, 1978, 61 (05) :1161-1167
[42]   Patent ductus arteriosus and neonatal death in prostaglandin receptor EP4-deficient mice [J].
Segi, E ;
Sugimoto, Y ;
Yamasaki, A ;
Aze, Y ;
Oida, H ;
Nishimura, T ;
Murata, T ;
Matsuoka, T ;
Ushikubi, F ;
Hirose, M ;
Tanaka, T ;
Yoshida, N ;
Narumiya, S ;
Ichikawa, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 246 (01) :7-12
[43]   An Augmented Negative Force-Frequency Relationship and Slowed Mechanical Restitution Are Associated With Increased Susceptibility to Drug-Induced Torsade de Pointes Arrhythmias in the Chronic Atrioventricular Block Dog [J].
Sprenkeler, David J. ;
Bossu, Alexandre ;
Beekman, Jet D. M. ;
Schoenmakers, Marieke ;
Vos, Marc A. .
FRONTIERS IN PHYSIOLOGY, 2018, 9
[44]   Co-culture of adipose-derived stem cells and endothelial cells in fibrin induces angiogenesis and vasculogenesis in a chorioallantoic membrane model [J].
Strassburg, Sandra ;
Nienhueser, Henrik ;
Stark, G. Bjoern ;
Finkenzeller, Guenter ;
Torio-Padron, Nestor .
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2016, 10 (06) :496-506
[45]   Specific expression of PP5/TFPI-2 mRNA by syncytiotrophoblasts in human placenta as revealed by in situ hybridization [J].
Udagawa, K ;
Miyagi, Y ;
Hirahara, F ;
Miyagi, E ;
Nagashima, Y ;
Minaguchi, H ;
Misugi, K ;
Yasumitsu, H ;
Miyazaki, K .
PLACENTA, 1998, 19 (2-3) :217-223
[46]   Canine congenital portosystemic shunts: Disconnections dissected [J].
Van den Bossche, L. ;
van Steenbeek, F. G. .
VETERINARY JOURNAL, 2016, 211 :14-20
[47]   The canine era: the rise of a biomedical model [J].
van Steenbeek, F. G. ;
Hytonen, M. K. ;
Leegwater, P. A. J. ;
Lohi, H. .
ANIMAL GENETICS, 2016, 47 (05) :519-527
[48]   Implication of Long noncoding RNAs in the endothelial cell response to hypoxia revealed by RNA-sequencing [J].
Voellenkle, C. ;
Garcia-Manteiga, J. M. ;
Pedrotti, S. ;
Perfetti, A. ;
De Toma, I. ;
Da Silva, D. ;
Maimone, B. ;
Greco, S. ;
Fasanaro, P. ;
Creo, P. ;
Zaccagnini, G. ;
Gaetano, C. ;
Martelli, F. .
SCIENTIFIC REPORTS, 2016, 6
[49]   Dendritic cells loading autologous tumor lysate promote tumor angiogenesis [J].
Yang, Yi ;
Lu, Jing ;
Liu, Hangfan ;
Jin, Guoguo ;
Bai, Ruihua ;
Li, Xiang ;
Wang, Dongyu ;
Zhao, Jimin ;
Huang, Youtian ;
Liu, Kangdong ;
Xing, Ying ;
Dong, Ziming .
TUMOR BIOLOGY, 2016, 37 (12) :15687-15695
[50]   Co-culture of mesenchymal stem cells with umbilical vein endothelial cells under hypoxic condition [J].
Zhang, Bo ;
Yang, Shuhua ;
Zhang, Yukun ;
Sun, Zhibo ;
Xu, Weihua ;
Ye, Shunan .
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2012, 32 (02) :173-180