Retinal Neuron Is More Sensitive to Blue Light-Induced Damage than Glia Cell Due to DNA Double-Strand Breaks

被引：29

作者：

Chen, Pei ^{[1
]}

Lai, Zhipeng ^{[1
]}

Wu, Yihui ^{[1
]}

Xu, Lijun ^{[1
]}

Cai, Xiaoxiao ^{[1
]}

Qiu, Jin ^{[1
]}

Yang, Panyang ^{[1
]}

Yang, Meng ^{[1
]}

Zhou, Pan ^{[1
]}

Zhuang, Jiejie ^{[1
]}

Ge, Jian ^{[1
]}

Yu, Keming ^{[1
]}

Zhuang, Jing ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510060, Guangdong, Peoples R China

来源：

CELLS | 2019年 / 8卷 / 01期

关键词：

blue light; retinal neuron; glia cell; DNA double strand breaks; MITOCHONDRIA; APOPTOSIS; REPAIR; EXPOSURE; PROTEIN; INVOLVEMENT; ISCHEMIA; STRESS; DEATH; RPE;

D O I：

10.3390/cells8010068

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Blue light is a major component of visible light and digital displays. Over-exposure to blue light could cause retinal damage. However, the mechanism of its damage is not well defined. Here, we demonstrate that blue light (900 lux) impairs cell viability and induces cell apoptosis in retinal neurocytes in vitro. A DNA electrophoresis assay shows severe DNA damage in retinal neurocytes at 2 h after blue light treatment. gamma-H2AX foci, a specific marker of DNA double-strand breaks (DSBs), is mainly located in the Map2-posotive neuron other than the glia cell. After assaying the expression level of proteins related to DNA repair, Mre11, Ligase IV and Ku80, we find that Ku80 is up-regulated in retinal neurocytes after blue light treatment. Interestingly, Ku80 is mainly expressed in glia fibrillary acidic protein (GFAP)-positive glia cells. Moreover, following blue light exposure in vivo, DNA DSBs are shown in the ganglion cell layer and only observed in Map2-positive cells. Furthermore, long-term blue light exposure significantly thinned the retina in vivo. Our findings demonstrate that blue light induces DNA DSBs in retinal neurons, and the damage is more pronounced compared to glia cells. Thus, this study provides new insights into the mechanisms of the effect of blue light on the retina.

引用

页数：14

共 37 条

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