The structure and function of the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Haemophilus influenzae

被引：51

作者：

Hennig, M ^{[1
]}

Dale, GE ^{[1
]}

D'Arcy, A ^{[1
]}

Danel, F ^{[1
]}

Fischer, S ^{[1
]}

Gray, CP ^{[1
]}

Jolidon, S ^{[1
]}

Müller, F ^{[1
]}

Page, MGP ^{[1
]}

Pattison, P ^{[1
]}

Oefner, C ^{[1
]}

机构：

[1] F Hoffmann La Roche & Co Ltd, Pharma Preclin Res, CH-4070 Basel, Switzerland

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1999年 / 287卷 / 02期

关键词：

HPPK; pterin; pyrophosphokinase; MAD; X-ray structure;

D O I：

10.1006/jmbi.1999.2623

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene encoding the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase of Haemophilus influenzae has been cloned and expressed in Escherichia coli. A complex of the purified protein with a substrate analog has been crystallized and its structure solved by multiple anomalous dispersion using phase information obtained from a single crystal of selenomethione-labeled protein. The enzyme folds into a four-stranded antiparallel beta-sheet flanked on one side by two alpha-helices and on the other by three consecutive alpha-helices, giving a novel beta(1)alpha(1)beta(2)beta(3)alpha(2)beta(4)alpha(3)alpha(4)alpha(5) polypeptide topology. The three-dimensional structure of a binary complex has been refined at 2.1 Angstrom resolution. The location of the substrate analog and a sulfate ion gives important insight into the molecular mechanism of the enzyme. (C) 1999 Academic Press.

引用

页码：211 / 219

页数：9

共 45 条

[41] PURIFICATION AND PARTIAL CHARACTERIZATION OF 7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN-PYROPHOSPHOKINASE AND 7,8-DIHYDROPTEROATE SYNTHASE FROM ESCHERICHIA-COLI MC4100 [J].

TALARICO, TL ;

DEV, IK ;

DALLAS, WS ;

FERONE, R ;

RAY, PH .

JOURNAL OF BACTERIOLOGY, 1991, 173 (21) :7029-7032

[42] CLONING, SEQUENCE-ANALYSIS, AND OVEREXPRESSION OF ESCHERICHIA-COLI FOLK, THE GENE CODING FOR 7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN-PYROPHOSPHOKINASE [J].

TALARICO, TL ;

RAY, PH ;

DEV, IK ;

MERRILL, BM ;

DALLAS, WS .

JOURNAL OF BACTERIOLOGY, 1992, 174 (18) :5971-5977

[43] PRIMARY STRUCTURE AND EXPRESSION OF THE DIHYDROPTEROATE SYNTHETASE GENE OF PLASMODIUM-FALCIPARUM [J].

TRIGLIA, T ;

COWMAN, AF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (15) :7149-7153

[44] THE MULTIFUNCTIONAL FOLIC-ACID SYNTHESIS FAS GENE OF PNEUMOCYSTIS-CARINII ENCODES DIHYDRONEOPTERIN ALDOLASE, HYDROXYMETHYLDIHYDROPTERIN PYROPHOSPHOKINASE AND DIHYDROPTEROATE SYNTHASE [J].

VOLPE, F ;

BALLANTINE, SP ;

DELVES, CJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 216 (02) :449-458

[45]

White TJ., 1990, PCR PROTOCOLS GUIDE, P315, DOI 10.1016/B978-0-12-372180

← 1 2 3 4 5 →