Dissecting the First Transcriptional Divergence During Human Embryonic Development

被引：61
作者：
Bai, Qiang [2 ,4 ]
Assou, Said [2 ]
Haouzi, Delphine [2 ,3 ]
Ramirez, Jean-Marie [2 ]
Monzo, Cecile [4 ]
Becker, Fabienne [3 ]
Gerbal-Chaloin, Sabine [2 ]
Hamamah, Samir [2 ,3 ,4 ,5 ]
De Vos, John [1 ,2 ,3 ,4 ,6 ]
机构：
[1] Hop St Eloi, Inst Res Biotherapy, F-34295 Montpellier 5, France
[2] INSERM, U1040, F-34000 Montpellier, France
[3] Hop St Eloi, CHU Montpellier, Inst Res Biotherapy, F-34000 Montpellier, France
[4] Univ Montpellier I, UFR Med, F-34000 Montpellier, France
[5] Hop Arnaud de Villeneuve, CHU Montpellier, ART PDG Dept, F-34000 Montpellier, France
[6] Hop St Eloi, CHU Montpellier, Unit Cellular Therapy, F-34000 Montpellier, France
来源：
STEM CELL REVIEWS AND REPORTS | 2012年 / 8卷 / 01期
关键词：
Pluripotent stem cells; Transcription factor; Transcriptional network; Transcriptome; Trophectoderm; DE-NOVO METHYLATION; STEM-CELL LINES; GENE-EXPRESSION; DNA METHYLATION; DIFFERENTIATION; PLACENTA; DNMT3L; TROPHECTODERM; ESTABLISHMENT; ANTIGENS;
D O I：
10.1007/s12015-011-9301-3
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The trophoblast cell lineage is specified early at the blastocyst stage, leading to the emergence of the trophectoderm and the pluripotent cells of the inner cell mass. Using a double mRNA amplification technique and a comparison with transcriptome data on pluripotent stem cells, placenta, germinal and adult tissues, we report here some essential molecular features of the human mural trophectoderm. In addition to genes known for their role in placenta (CGA, PGF, ALPPL2 and ABCG2), human trophectoderm also strongly expressed Laminins, such as LAMA1, and the GAGE Cancer/Testis genes. The very high level of ABCG2 expression in trophectoderm, 7.9-fold higher than in placenta, suggests a major role of this gene in shielding the very early embryo from xenobiotics. Several genes, including CCKBR and DNMT3L, were specifically up-regulated only in trophectoderm, indicating that the trophoblast cell lineage shares with the germinal lineage a transient burst of DNMT3L expression. A trophectoderm core transcriptional regulatory circuitry formed by 13 tightly interconnected transcription factors (CEBPA, GATA2, GATA3, GCM1, KLF5, MAFK, MSX2, MXD1, PPARD, PPARG, PPP1R13L, TFAP2C and TP63), was found to be induced in trophectoderm and maintained in placenta. The induction of this network could be recapitulated in an in vitro trophoblast differentiation model.
引用
收藏
页码：150 / 162
页数：13
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