Crystallization and X-ray data collection of HP0902 from Helicobacter pylori 26695

被引:0
作者
Sim, Dae-Won [2 ]
Song, Jung Hyun [1 ]
Lee, Woo Cheol [1 ]
Lee, Yoo-Sup [2 ]
Kim, Hye-Yeon [1 ]
Won, Hyung-Sik [2 ]
机构
[1] Korea Basic Sci Inst, Div Magnet Resonance Res, Ochang 363883, Chungbuk, South Korea
[2] Konkuk Univ, Dept Biotechnol, Coll Biomed & Hlth Sci, Chungju 380701, Chungbuk, South Korea
来源
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2011年 / 67卷
关键词
HP0902; Helicobacter pylori; His6; tag; cupin superfamily; CUPIN SUPERFAMILY;
D O I
10.1107/S1744309111039315
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
HP0902 from Helicobacter pylori 26695 belongs to the cupin superfamily of proteins, which encompasses proteins with a great diversity in function. In this work, two types of recombinant HP0902 protein were crystallized: one with an N-terminal His6 tag (H6HP0902) and the other with a C-terminal His6 tag (HP0902H6). The H6HP0902 crystal diffracted to 1.40 angstrom resolution and belonged to space group P21, with unit-cell parameters a = 33.5, b = 78.6, c = 41.4 angstrom. The HP0902H6 crystal belonged to space group P43212 or P41212 and diffracted to 2.5 angstrom resolution, with unit-cell parameters a = b = 50.4, c = 142.0 angstrom.
引用
收藏
页码:1619 / 1622
页数:4
相关论文
共 15 条
  • [1] Cupins: the most functionally diverse protein superfamily?
    Dunwell, JM
    Purvis, A
    Khuri, S
    [J]. PHYTOCHEMISTRY, 2004, 65 (01) : 7 - 17
  • [2] Scaling and assessment of data quality
    Evans, P
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2006, 62 : 72 - 82
  • [3] The Pfam protein families database
    Finn, Robert D.
    Mistry, Jaina
    Tate, John
    Coggill, Penny
    Heger, Andreas
    Pollington, Joanne E.
    Gavin, O. Luke
    Gunasekaran, Prasad
    Ceric, Goran
    Forslund, Kristoffer
    Holm, Liisa
    Sonnhammer, Erik L. L.
    Eddy, Sean R.
    Bateman, Alex
    [J]. NUCLEIC ACIDS RESEARCH, 2010, 38 : D211 - D222
  • [4] Pleiotropic Actions of Helicobacter pylori Vacuolating Cytotoxin, VacA
    Isomoto, Hajime
    Moss, Joel
    Hirayama, Toshiya
    [J]. TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 2010, 220 (01) : 3 - 14
  • [5] Proteins released by Helicobacter pylori in vitro
    Kim, N
    Weeks, DL
    Shin, JM
    Scott, DR
    Young, MK
    Sachs, G
    [J]. JOURNAL OF BACTERIOLOGY, 2002, 184 (22) : 6155 - 6162
  • [6] LESLIE AGW, 1992, JNT CCP4 ESF EA CBM, V26
  • [7] SOLVENT CONTENT OF PROTEIN CRYSTALS
    MATTHEWS, BW
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) : 491 - +
  • [8] Mills ENC, 2002, BIOCHEM SOC T, V30, P925, DOI 10.1042/BST0300925
  • [9] The fdxA ferredoxin gene can down-regulate frxA nitroreductase gene expression and is essential in many strains of Helicobacter pylori
    Mukhopadhyay, AK
    Jeong, JY
    Dailidiene, D
    Hoffman, PS
    Berg, DE
    [J]. JOURNAL OF BACTERIOLOGY, 2003, 185 (09) : 2927 - 2935
  • [10] NAM WH, 2007, KOREAM J LIFE SCI, V17, P723