Role of dissolved oxygen availability on lactobionic acid production from whey by Pseudomonas taetrolens

被引：35

作者：

Alonso, Saul ^{[1
]}

Rendueles, Manuel ^{[1
]}

Diaz, Mario ^{[1
]}

机构：

[1] Univ Oviedo, Fac Chem, Dept Chem Engn & Environm Technol, E-33071 Oviedo, Spain

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 109卷

关键词：

Lactobionic acid; Whey; Pseudomonas taetrolens; Dissolved oxygen; Oxygen uptake rate; MOLECULAR-WEIGHT; AGITATION; FERMENTATION; SORBITOL; CULTURES; ALGINATE; SPEED;

D O I：

10.1016/j.biortech.2012.01.045

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The influence of dissolved oxygen availability on cell growth and lactobionic acid production from whey by Pseudomonas taetrolens has been investigated for the first time. Results from pH-shift bioreactor cultivations have shown that high agitation rate schemes stimulated cell growth, increased pH-shift values and the oxygen uptake rate by cells, whereas lactobionic acid production was negatively affected. Conversely, higher aeration rates than 1.5 Lpm neither stimulated cell growth nor lactobionic acid production (22% lower for an aeration rate of 2 Lpm). Overall insights into bioprocess performance enabled the implementation of 350 rpm as the optimal agitation strategy during cultivation, which increased lactobionic productivity 1.2-fold (0.58-0.7 g/L h) compared to that achieved at 1000 rpm. Oxygen supply has been shown to be a key bioprocess parameter for enhanced overall efficiency of the system, representing essential information for the implementation of lactobionic acid production at a large scale. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：140 / 147

页数：8

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