Application of immunoprinting for assessment of fibroblast secretory heterogeneity

被引:8
作者
DongariBagtzoglou, AI [1 ]
Ebersole, JL [1 ]
机构
[1] UNIV TEXAS,HLTH SCI CTR,DEPT PERIODONT,SAN ANTONIO,TX 78284
关键词
cytokine; adherent cell culture; fibroblast; immunoprinting;
D O I
10.1016/S0022-1759(96)00147-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Fibroblasts are capable of secreting a number of inflammatory mediators and cytokines and may exhibit a marked heterogeneity in this capacity. The relative frequency of cytokine-secretin fibroblasts in chronic inflammatory connective tissue disorders may affect the amount of these molecules secreted locally and dictate the intensity or chronicity of the disease process. We have devised a simple, in situ immunodetection method for assessment of the frequency of actively secreting cells in adherent cell cultures. Our technique is based on the principle of immunoprinting and is coupled with an enzyme-linked immunodetection system. The methodology, sensitivity, specificity and accuracy of this technique are described.
引用
收藏
页码:145 / 154
页数:10
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