Study on the interaction between ICT fluorescence probe and bovine serum albumins

被引：0

作者：

Liu Yu-fang ^{[1
,2
]}

Li Jian-qing ^{[1
,2
]}

Xu Zhi-cheng ^{[1
]}

Wei Yan-li ^{[1
]}

Shuang Shao-min ^{[1
]}

Dong Chuan ^{[1
]}

机构：

[1] Shanxi Univ, Dept Chem, Taiyuan 030006, Peoples R China

[2] Jinzhong Univ, Dept Chem, Yuci 030600, Peoples R China

来源：

SPECTROSCOPY AND SPECTRAL ANALYSIS | 2008年 / 28卷 / 04期

关键词：

1-keto-2-(p-dimethylaminobenzal)-tetrohydro naphthalene; bovine serum albumin; fluorescence quenching; hydrophobic interaction;

D O I：

暂无

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The present article studied the interaction between intramolecular charge transfer fluorescence probe-1-keto-2-(p-dimethylaminobenzal)-tetrohydronaphthalene (KDTN) and bovine serum albumins (BSA). With the concentration of KDTN increasing, the fluorescence of BSA rapidly quenched and the fluorescence peak gradually blue-shifted. The result indicated that they were bound mainly by hydrophobic interaction. The binding sites is 0.94 (3 degrees C) and the equilibrium constant K is 3.27 x 10(4) L . mol(-1). Temperature increment is advantageous to the combination. It is a single static quenching process that the fluorescence of BSA quenches, which is induced by the combination of KDTN and BSA. Further study showed that different substances had different effects on the combination of KDTN and BSA.

引用

页码：887 / 890

页数：4

共 9 条

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