Mixotrophic cultivation of Chlorella pyrenoidosa under sulfadiazine stress: High-value product recovery and toxicity tolerance evaluation

Sulfadiazine (SDZ) as a common sulfonamide antibiotic is frequently detected in wastewater, but there is little information on the high-value product recovery and toxicity tolerance evaluation of mixotrophic microalgae under SDZ stress. In this study, effects of SDZ on growth, photosynthesis, cellular damage, antioxidant capacity and intracellular biochemical components of Chlorella pyrenoidosa were investigated. Results showed that the growth of C. pyrenoidosa was inhibited by about 20% under high SDZ stress, but there was little impact on photosynthesis. Cellular damage and antioxidant capacity were evaluated using malondialdehyde (MDA) content and superoxide dismutase (SOD) activity to further explain the toxicity tolerance of mixotrophic microalgae. The SDZ stress not only increased lipid and carbohydrate content, respectively attaining to the maximum of 390.0 and 65.4 mg/L, but also improved the biodiesel quality of C. pyrenoidosa. The findings show the potential of mixotrophic microalgae for biodiesel production and wastewater treatment.