A 500-kb sequence-ready cosmid contig and transcript map of the MEN1 region on 11q13

被引：4

作者：

Bergman, L

Silins, G

Grimmond, S

Hummerich, H

Stewart, C

Little, P

Hayward, N ^{[1
]}

机构：

[1] PO Royal Brisbane Hosp, Queensland Inst Med Res, Joint Expt Oncol Program, Queensland Canc Fund,Res Unit, Herston, Qld 4029, Australia

[2] MRC, Mammalian Genet Unit, Harwell OX11 0RD, Oxon, England

[3] Univ London Imperial Coll Sci Technol & Med, London SW7 2AZ, England

来源：

GENOMICS | 1999年 / 55卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1006/geno.1998.5625

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have generated a transcript map of an approximately 1.2-Mb region from human chromosome band 11q13 between the loci VEGFB and CAPN1, which flank the multiple endocrine neoplasia type 1 (MEN 1) locus. In total, we isolated 144 cosmids from this region and generated a sequence-ready cosmid contig of the approximately 500-kb region between the neurexin locus and D11S2196E. We identified 54 genes/ESTs by sample sequencing and have constructed a transcript map of this region. Genes were found to be clustered in three regions, and one of these genes was identical to the recently identified MEN1 locus. Relative to the latter, we have mapped the positions of 13 known genes, 18 genes which show homology to genes from humans or other organisms, and 22 genes/ESTs that appear novel. In addition, we have ascertained the directions of transcription of some of these genes and have determined intergenic distances between many loci. Full characterization of some of these genes, as well as the novel ESTs, will be useful in identifying candidate genes for other diseases known to map to this chromosomal region. (C) 1999 Academic Press.

引用

页码：49 / 56

页数：8

共 31 条

[1] Germline mutations of the MEN1 gene in familial multiple endocrine neoplasia type 1 and related states [J].

Agarwal, SK ;

Kester, MB ;

Debelenko, LV ;

Heppner, C ;

EmmertBuck, MR ;

Skarulis, MC ;

Doppman, JL ;

Kim, YS ;

Lubensky, IA ;

Zhuang, ZP ;

Green, JS ;

Guru, SC ;

Manickam, P ;

Olufemi, SE ;

Liotta, LA ;

Chandrasekharappa, SC ;

Collins, FS ;

Spiegel, AM ;

Burns, AL ;

Marx, SJ .

HUMAN MOLECULAR GENETICS, 1997, 6 (07) :1169-1175

[2] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[3]

BERGHAMMER H, 1993, BIOTECHNIQUES, V14, P522

[4] Positional cloning of the gene for multiple endocrine neoplasia-type 1 [J].

Chandrasekharappa, SC ;

Guru, SC ;

Manickam, P ;

Olufemi, SE ;

Collins, FS ;

EmmertBuck, MR ;

Debelenko, LV ;

Zhuang, ZP ;

Lubensky, IA ;

Liotta, LA ;

Crabtree, JS ;

Wang, YP ;

Roe, BA ;

Weisemann, J ;

Boguski, MS ;

Agarwal, SK ;

Kester, MB ;

Kim, YS ;

Heppner, C ;

Dong, QH ;

Spiegel, AM ;

Burns, AL ;

Marx, SJ .

SCIENCE, 1997, 276 (5311) :404-407

[5] Definition of the minimal MEN1 candidate area based on a 5-Mb integrated map of proximal 11q13 - The European consortium on MEN1 [J].

Courseaux, A ;

Grosgeorge, J ;

Gaudray, P ;

Pannett, AAJ ;

Forbes, SA ;

Williamson, C ;

Bassett, D ;

Thakker, RV ;

Teh, BT ;

Farnebo, F ;

Shepherd, J ;

Skogseid, B ;

Larsson, C ;

Giraud, S ;

Zhang, CX ;

Salandre, J ;

Calender, A .

GENOMICS, 1996, 37 (03) :354-365

[6] CPG ISLANDS AND GENES [J].

CROSS, SH ;

BIRD, AP .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1995, 5 (03) :309-314

[7] Multiple facial angiofibromas and collagenomas in patients with multiple endocrine neoplasia type 1 [J].

Darling, TN ;

Skarulis, MC ;

Steinberg, SM ;

Marx, SJ ;

Spiegel, AM ;

Turner, M .

ARCHIVES OF DERMATOLOGY, 1997, 133 (07) :853-857

[8] A GENOME-WIDE SEARCH FOR HUMAN TYPE-1 DIABETES SUSCEPTIBILITY GENES [J].

DAVIES, JL ;

KAWAGUCHI, Y ;

BENNETT, ST ;

COPEMAN, JB ;

CORDELL, HJ ;

PRITCHARD, LE ;

REED, PW ;

GOUGH, SCL ;

JENKINS, SC ;

PALMER, SM ;

BALFOUR, KM ;

ROWE, BR ;

FARRALL, M ;

BARNETT, AH ;

BAIN, SC ;

TODD, JA .

NATURE, 1994, 371 (6493) :130-136

[9] Identification of MEN1 gene mutations in sporadic carcinoid tumors of the lung [J].

Debelenko, LV ;

Brambilla, E ;

Agarwal, SK ;

Swalwell, JI ;

Kester, MB ;

Lubensky, IA ;

Zhuang, ZP ;

Guru, SC ;

Manickam, P ;

Olufemi, SE ;

Chandrasekharappa, SC ;

Crabtree, JS ;

Kim, YS ;

Heppner, C ;

Burns, AL ;

Spiegel, AM ;

Marx, SJ ;

Liotta, LA ;

Collins, FS ;

Travis, WD ;

EmmertBuck, MR .

HUMAN MOLECULAR GENETICS, 1997, 6 (13) :2285-2290

[10]

EmmertBuck MR, 1997, CANCER RES, V57, P1855

← 1 2 3 4 →