Greatly Enhanced Removal of Volatile Organic Carcinogens by a Genetically Modified Houseplant, Pothos Ivy (Epipremnum aureum) Expressing the Mammalian Cytochrome P450 2e1 Gene

被引：26

作者：

Zhang, Long ^{[1
]}

Routsong, Ryan ^{[1
]}

Strand, Stuart E. ^{[1
]}

机构：

[1] Univ Washington, Dept Civil & Environm Engn, Box 355014, Seattle, WA 98195 USA

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2019年 / 53卷 / 01期

关键词：

GREEN FLUORESCENT PROTEIN; FORMALDEHYDE DEHYDROGENASE; AIR-POLLUTANTS; TRANSFORMATION; DETOXIFICATION; METABOLISM; PLANTS; PHYTOREMEDIATION; OVEREXPRESSION; DEGRADATION;

D O I：

10.1021/acs.est.8b04811

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The indoor air in urban homes of developed countries is usually contaminated with significant levels of volatile organic carcinogens (VOCs), such as formaldehyde, benzene, and chloroform. There is a need for a practical, sustainable technology for the removal of VOCs in homes. Here we show that a detoxifying transgene, mammalian cytochrome P450 2e1 can be expressed in a houseplant, Epipremnum aureum, pothos ivy, and that the resulting genetically modified plant has sufficient detoxifying activity against benzene and chloroform to suggest that biofilters using transgenic plants could remove VOCs from home air at useful rates.

引用

页码：325 / 331

页数：7

共 34 条

[11] Risk assessment of exposure to volatile organic compounds in different indoor environments
Guo, H
Lee, SC
Chan, LY
Li, WM
[J]. ENVIRONMENTAL RESEARCH, 2004, 94 (01) : 57 - 66
[12] Green fluorescent protein as a marker for expression of a second gene in transgenic plants
Harper, BK
Mabon, SA
Leffel, SM
Halfhill, MD
Richards, HA
Moyer, KA
Stewart, CN
[J]. NATURE BIOTECHNOLOGY, 1999, 17 (11) : 1125 - 1129
[13] Characteristics and health impacts of VOCs and carbonyls associated with residential cooking activities in Hong Kong
Huang, Yu
Ho, Steven Sai Hang
Ho, Kin Fai
Lee, Shun Cheng
Yu, Jian Zhen
Louie, Peter K. K.
[J]. JOURNAL OF HAZARDOUS MATERIALS, 2011, 186 (01) : 344 - 351
[14] Degradation of low molecular weight volatile organic compounds by plants genetically modified with mammalian cytochrome P450 2E1
James, C. Andrew
Xin, Gang
Doty, Sharon L.
Strand, Stuart E.
[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2008, 42 (01) : 289 - 293
[15] ACTIVITY PATTERNS OF CALIFORNIANS - USE OF AND PROXIMITY TO INDOOR POLLUTANT SOURCES
JENKINS, PL
PHILLIPS, TJ
MULBERG, EJ
HUI, SP
[J]. ATMOSPHERIC ENVIRONMENT PART A-GENERAL TOPICS, 1992, 26 (12): : 2141 - 2148
[16] Genetic transformation of golden pothos (Epipremnum aureum) mediated by Agrobacterium tumefaciens
Kotsuka, Katsutoshi
Tada, Yuichi
[J]. PLANT CELL TISSUE AND ORGAN CULTURE, 2008, 95 (03) : 305 - 311
[17] A Field Trial of TCE Phytoremediation by Genetically Modified Poplars Expressing Cytochrome P450 2E1
Legault, Emily K.
James, C. Andrew
Stewart, Keith
Muiznieks, Indulis
Doty, Sharon L.
Strand, Stuart E.
[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2017, 51 (11) : 6090 - 6099
[18] Ranking cancer risks of organic hazardous air pollutants in the United States
Loh, Miranda M.
Levy, Jonathan I.
Spengler, John D.
Houseman, E. Andres
Bennett, Deborah H.
[J]. ENVIRONMENTAL HEALTH PERSPECTIVES, 2007, 115 (08) : 1160 - 1168
[19] Gateway-compatible vectors for high-throughput gene functional analysis in switchgrass (Panicum virgatum L.) and other monocot species
Mann, David G. J.
LaFayette, Peter R.
Abercrombie, Laura L.
King, Zachary R.
Mazarei, Mitra
Halter, Mathew C.
Poovaiah, Charleson R.
Baxter, Holly
Shen, Hui
Dixon, Richard A.
Parrott, Wayne A.
Stewart, C. Neal, Jr.
[J]. PLANT BIOTECHNOLOGY JOURNAL, 2012, 10 (02) : 226 - 236
[20] Stable transformation of Theobroma cacao L. and influence of matrix attachment regions on GFP expression
Maximova, S
Miller, C
de Mayolo, GA
Pishak, S
Young, A
Guiltinan, MJ
[J]. PLANT CELL REPORTS, 2003, 21 (09) : 872 - 883

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