Plasmon-Enhanced Colorimetric ELISA with Single Molecule Sensitivity

被引：153

作者：

Chen, Si ^{[1
]}

Svedendahl, Mikael ^{[1
]}

Van Duyne, Richard P. ^{[2
]}

Kall, Mikael ^{[1
]}

机构：

[1] Chalmers, Dept Appl Phys, S-41296 Gothenburg, Sweden

[2] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA

来源：

NANO LETTERS | 2011年 / 11卷 / 04期

基金：

美国国家科学基金会; 瑞典研究理事会;

关键词：

Surface plasmon; ELISA; biosensor; single molecules; single particles; spectra imaging; NANOPARTICLES; BIOSENSORS; LITHOGRAPHY; PEROXIDASE; RESONANCE; PROTEINS;

D O I：

10.1021/nl2006092

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Robust but ultrasensitive biosensors with a capability of detecting low abundance biomarkers could revolutionize clinical diagnostics and enable early detection of cancer, neurological diseases, and infections. We utilized a combination of localized surface plasmon resonance (LSPR) refractive index sensing and the well-known enzyme-linked immunosorbent assay to develop a simple colorimetric biosensing methodology with single molecule sensitivity. The technique is based on spectral imaging of a large number of isolated gold nanoparticles. Each particle binds a variable number of horseradish peroxidase (HRP) enzyme molecules that catalyze a localized precipitation reaction at the particle surface. The enzymatic reaction dramatically amplifies the shift of the LSPR scattering maximum, lambda(max), and makes it possible to detect the presence of only one or a few HRP molecules per particle.

引用

页码：1826 / 1830

页数：5

共 26 条

[1]

ADAMCZYK Z, 1994, KINETICS LOCALIZED A

[2] Biosensing with plasmonic nanosensors [J].