The rhizosphere of Sulla spinosissima growing in abandoned mining soils is a reservoir of heavy metals tolerant plant growth-promoting rhizobacteria

被引:7
作者
Oubohssaine, Malika [1 ]
Dahmani, Ikram [1 ]
Sbabou, Laila [1 ]
Bruneel, Odile [2 ]
Aurag, Jamal [1 ]
机构
[1] Mohammed V Univ Rabat, Fac Sci, Ctr Plant & Microbial Biotechnol, Microbiol & Mol Biol Team,Biodivers & Environm, Ave Ibn Battouta,BP 1014, Rabat 10000, Morocco
[2] Univ Montpellier, Lab HydroSci Montpellier, UMR5559 CNRS IRD UM, CC0057 MSE,16 Rue Auguste Broussonet, F-34090 Montpellier, France
来源
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2022年 / 39卷
关键词
Abandoned mining site; Cultivable bacteria; Sulla spinosissima rhizosphere; PGP traits; Antifungal activity; Heavy metal tolerance; ANTIFUNGAL ACTIVITY; RIBOSOMAL-RNA; BACTERIA; PHYTOREMEDIATION; CONTAMINATION; BIOCONTROL; ACCUMULATION; SIDEROPHORE; EFFICIENCY; DIVERSITY;
D O I
10.1016/j.bcab.2021.102236
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Heavy metals tolerant soil bacteria are known to play essential roles in biogeochemical cycles, biotransformation of metals, bioremediation processes and, plant adaptation. The objective of this study was to isolate and characterize the bacterial strains associated with Sulla spinosissima, a native legume species growing in three abandoned mining sites situated in the Oujda region (East Morocco). Globally, more than 370 bulk and rhizospheric soil bacteria were isolated. Their identification by 16S rDNA sequencing showed that dominant phyla were Firmicutes, Actinobacteria, and Proteobacteria, while at the genus level Bacillus dominated, followed by Stenotrophomonas, Arthrobacter, and Rhodococcus. All the soils contained strains possessing plant growth-promoting traits. The best-performing strains were LMR283 for auxin production (145 mu g mL(-1)), LMR291 for phosphate solubilization (67.6 mg L-1), LMR280 for siderophore production (92.4%) and LMR326 for ACC deaminase activity (105 nmoL alpha-ketobutyrate mg(-1) h(-1)). Among tolerant PGP bacteria, 17 isolates showed antagonistic activity against the pathogen Fusarium oxysporum and 26 produced lytic enzymes. It was relevant that the rhizospheric soils prospected compared to bulk soils contained more interesting isolates for all the studied properties, in particular for soils sampled from Oued El Heimer and Sidi Boubker sites (respectively 79 and 63% of performing strains). Data presented here indicate that Sulla spinosissima growing in heavy metal soils is associated with multifarious active bacterial populations that probably sustain plant tolerance and growth under the prevailing stressful conditions. Superior strains identified are good candidates to be used with selected plants in rehabilitation programs in the contaminated ecosystems.
引用
收藏
页数:16
相关论文
共 50 条
  • [41] Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Coffee Plantation Soils and Its Influence on Maize Growth
    Waday, Yasin Ahmed
    Girma Aklilu, Ermias
    Bultum, Mohammed Seid
    Ramayya Ancha, Venkata
    Beyene, Dejene
    APPLIED AND ENVIRONMENTAL SOIL SCIENCE, 2022, 2022
  • [42] Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil
    Mustapha, Zakiah
    Othman, Radziah
    Nasir, Nik Nurnaeimah N. I. K. M. U. H. A. M. M. A. D.
    Zawawi, Dhiya Dalila
    Lah, Mohd Khairi C. H. E.
    Juahir, Hafizan
    FRONTIERS OF AGRICULTURAL SCIENCE AND ENGINEERING, 2024, 11 (03) : 499 - 513
  • [43] Plant growth-promoting rhizobacteria associated to candelilla rhizosphere (Euphorbia antisyphilitica) and its effects on Arabidopsis thaliana seedlings
    Salazar-Ramirez, Maria T.
    Saenz-Mata, Jorge
    Preciado-Rangel, Pablo
    Fortis-Hernandez, Manuel
    Rueda-Puente, Edgar O.
    Yescas-Coronado, Pablo
    Orozco-Vidal, Jorge A.
    NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA, 2021, 49 (02) : 1 - 17
  • [44] Inoculation with plant growth-promoting rhizobacteria improves seagrass Thalassia hemprichii photosynthesis performance and shifts rhizosphere microbiome
    Zhou, Weiguo
    Ling, Juan
    Shen, Xiaomei
    Xu, Zhimeng
    Yang, Qingsong
    Yue, Weizhong
    Liu, Hongbin
    Suo, Anning
    Dong, Junde
    MARINE ENVIRONMENTAL RESEARCH, 2024, 193
  • [45] Effects of Plant Growth-Promoting Rhizobacteria and N Source on Plant Growth and N and P Uptake by Tomato Grown on Calcareous Soils
    Fan, Xiaohui
    Zhang, Shouan
    Mo, Xiaodan
    Li, Yuncong
    Fu, Yuqing
    Liu, Zhiguang
    PEDOSPHERE, 2017, 27 (06) : 1027 - 1036
  • [46] Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils
    Habibi, Safiullah
    Djedidi, Salem
    Ohkama-Ohtsu, Naoko
    Sarhadi, Wakil Ahmad
    Kojima, Katsuhiro
    Rallos, Roland V.
    Ramirez, Maria Daniela Artigas
    Yamaya, Hiroko
    Sekimoto, Hitoshi
    Yokoyama, Tadashi
    MICROBES AND ENVIRONMENTS, 2019, 34 (04) : 347 - 355
  • [47] Fungicide-Tolerant Plant Growth-Promoting Rhizobacteria Mitigate Physiological Disruption of White Radish Caused by Fungicides Used in the Field Cultivation
    Khan, Sadaf
    Shahid, Mohammad
    Khan, Mohammad Saghir
    Syed, Asad
    Bahkali, Ali H.
    Elgorban, Abdallah M.
    Pichtel, John
    INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2020, 17 (19) : 1 - 26
  • [48] Plant growth-promoting fungi and rhizobacteria control Fusarium damping-off in Mason pine seedlings by impacting rhizosphere microbes and altering plant physiological pathways
    Yu, Cun
    Lv, Jun
    Xu, Hongyun
    PLANT AND SOIL, 2024, 499 (1-2) : 503 - 519
  • [49] Real-Time PCR (qtPCR) to Discover the Fate of Plant Growth-Promoting Rhizobacteria (PGPR) in Agricultural Soils
    Iosa, Ilenia
    Agrimonti, Caterina
    Marmiroli, Nelson
    MICROORGANISMS, 2024, 12 (05)
  • [50] Screening and Whole-Genome Sequencing of Two Streptomyces Species from the Rhizosphere Soil of Peony Reveal Their Characteristics as Plant Growth-Promoting Rhizobacteria
    Wang, Chengqiang
    Wang, Yun
    Ma, Jinjin
    Hou, Qihui
    Liu, Kai
    Ding, Yanqin
    Du, Binghai
    BIOMED RESEARCH INTERNATIONAL, 2018, 2018