Production, purification and identification of fructooligosaccharides produced by β-fructofuranosidase from Aspergillus niger IMI303386

被引：35
|
作者：
Nguyen, QD
Mattes, F
Hoschke, A
Rezessy-Szabó, J
Bhat, MK
机构：
[1] Inst Food Res, Reading Lab, Food Macromol Sci Dept, Reading RG6 6BZ, Berks, England
[2] Univ Hort & Food Ind, Dept Brewery & Distillery, H-1118 Budapest, Hungary
来源：
BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 03期
基金：
匈牙利科学研究基金会; 英国生物技术与生命科学研究理事会;
关键词：
beta-fructofuranosidase; inulinase; FOS; kestose; nystose;
D O I：
10.1023/A:1005429525865
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aspergillus niger IMI 303386 produced higher levels of intra- and extracellular beta-fructofuranosidase and inulinase on inulin than on sucrose. Intracellular beta-fructofuranosidase from sucrose medium catalysed the best transfructosylation reaction. The concentration of fructooligosaccharides (FOS) reached a maximum in 72 h with 25% (w/v) sucrose. The FOS were purified and the main products were kestose and nystose. Inulinase hydrolysed inulin in an exofashion and released mainly fructose.
引用
收藏
页码：183 / 186
页数：4
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