Potential Use of Multiplex PCR in Diagnosis of Tuberculous Meningitis

被引:0
作者
Philip, N. [1 ]
Wilkes, C. [2 ,3 ]
Yeo, T. W. [4 ]
Rajahram, G. S. [5 ]
William, T. [6 ]
John, D., V [1 ]
机构
[1] Univ Malaysia Sabah, Biotechnol Res Inst, Sabah, Malaysia
[2] Sabah Menzies Sch Hlth, Infect Dis Soc, Kota Kinabalu, Sabah, Malaysia
[3] Clin Res Unit, Kota Kinabalu, Sabah, Malaysia
[4] Nanyang Technol Univ, Singapore, Singapore
[5] Queen Elizabeth Hosp, Kota Kinabalu, Sabah, Malaysia
[6] Jesselton Med Ctr, Kota Kinabalu, Sabah, Malaysia
关键词
POLYMERASE-CHAIN-REACTION; EXTRAPULMONARY TUBERCULOSIS; MYCOBACTERIUM-TUBERCULOSIS; CEREBROSPINAL-FLUID; RAPID DIAGNOSIS; MTB/RIF; MPB-64; IS6110; ASSAY; DNA;
D O I
暂无
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Accurate and rapid diagnosis of tuberculous meningitis (TBM) is important for early administration of treatment. In this study, we have evaluated the diagnostic value of smear, culture, multiplex PCR and GeneXpert MTB/RIF to detect M. tuberculosis in cerebrospinal fluid (CSF) samples from patients with suspected TBM registered in Queen Elizabeth Hospital, Kota Kinabalu, Sabah, Malaysia. Of the 55 CSF samples collected, 12 (21.8%) samples were positive by multiplex PCR, 3 (5.4%) by GeneXpert MTB/RIF and only 1 (1.8%) by smear and culture. Multiplex PCR showed higher sensitivity to detect M. tuberculosis in patients with suspected TBM and has the potential to be used as a diagnostic method.
引用
收藏
页码:870 / 876
页数:7
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