Size characterization of magnetic cell sorting microbeads using flow field-flow fractionation and photon correlation spectroscopy

被引:10
作者
Williams, SKR [1 ]
Lee, H [1 ]
Turner, MM [1 ]
机构
[1] Colorado Sch Mines, Dept Chem & Geochem, Golden, CO 80401 USA
关键词
field-flow fractionation; photon correlation spectroscopy;
D O I
10.1016/S0304-8853(98)00551-4
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Flow field-flow fractionation (FFF) and photon correlation spectroscopy (PCS) were used to determine the size distribution of magnetic cell sorting (MACS) microbeads. The nominal 50 nm microbeads, when coupled to monoclonal Anti-Fluorescein isothiocyanate (FITC), have an average diameter of 125 nm. FFF results showed that two different batches of microbeads had similar average diameters. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:248 / 253
页数:6
相关论文
共 50 条
  • [31] Flow field-flow fractionation and characterization of ionic and neutral polysaccharides of vegetable and microbial origin
    Benincasa, MA
    Cartoni, G
    Delle Fratte, C
    JOURNAL OF CHROMATOGRAPHY A, 2002, 967 (02) : 219 - 234
  • [32] Comparison of Size-Exclusion Chromatography and Flow Field-Flow Fractionation for Separation of Whey Proteins
    Kang, Da Young
    Moon, Jae Mi
    Lee, Seungho
    BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2011, 32 (04): : 1315 - 1320
  • [33] Influence of ionic strength, sample size, and flow conditions on the retention behavior of pullulan in flow field-flow fractionation
    Benincasa, MA
    Delle Fratte, C
    JOURNAL OF CHROMATOGRAPHY A, 2004, 1046 (1-2) : 175 - 184
  • [34] Characterization of a microscale thermal-electrical field-flow fractionation system
    Sant, Himanshu J.
    Gale, Bruce K.
    JOURNAL OF CHROMATOGRAPHY A, 2012, 1225 : 174 - 181
  • [35] An overview on field-flow fractionation techniques and their applications in the separation and characterization of polymers
    Messaud, Fathi A.
    Sanderson, Ron D.
    Runyon, J. Ray
    Otte, Tino
    Pasch, Harald
    Williams, S. Kim Ratanathanawongs
    PROGRESS IN POLYMER SCIENCE, 2009, 34 (04) : 351 - 368
  • [36] DETERMINATION OF PARTICLE-SIZE DISTRIBUTION BY GRAVITATIONAL FIELD-FLOW FRACTIONATION - DIMENSIONAL CHARACTERIZATION OF SILICA PARTICLES
    RESCHIGLIAN, P
    TORSI, G
    CHROMATOGRAPHIA, 1995, 40 (7-8) : 467 - 473
  • [37] Characterization of titanium dioxide nanoparticles imprinted for tyrosine by flow field-flow fractionation and spectrofluorimetric analysis
    Zattoni, Andrea
    Reschiglian, Pierluigi
    Montalti, Marco
    Zaccheroni, Nelsi
    Prodi, Luca
    Picca, Rosaria Anna
    Malitesta, Cosimino
    INORGANICA CHIMICA ACTA, 2007, 360 (03) : 1063 - 1071
  • [38] Size fractionation and size characterization of nanoemulsions of lipid droplets and large unilamellar lipid vesicles by asymmetric-flow field-flow fractionation/multi-angle light scattering and dynamic light scattering
    Vezocnik, Valerija
    Rebolj, Katja
    Sitar, Simona
    Ota, Katja
    Tusek-Znidaric, Magda
    Strus, Jasna
    Sepcic, Kristina
    Pahovnik, David
    Macek, Peter
    Zagar, Ema
    JOURNAL OF CHROMATOGRAPHY A, 2015, 1418 : 185 - 191
  • [39] CHARACTERIZATION OF NEAR-WALL HYDRODYNAMIC LIFT FORCES USING SEDIMENTATION FIELD-FLOW FRACTIONATION
    WILLIAMS, PS
    KOCH, T
    GIDDINGS, JC
    CHEMICAL ENGINEERING COMMUNICATIONS, 1992, 111 : 121 - 147
  • [40] Separation and characterization of nanoparticles in complex food and environmental samples by field-flow fractionation
    von der Kammer, Frank
    Legros, Samuel
    Larsen, Erik H.
    Loeschner, Katrin
    Hofmann, Thilo
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2011, 30 (03) : 425 - 436